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Bioethanol Production from Eucalyptus camaldulensis Wood Waste Using Bacillus subtilis and Escherichia coli Isolated from Soil in Afaka Forest Reserve, Kaduna State Nigeria

Received: 29 January 2015     Accepted: 8 February 2015     Published: 30 March 2015
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Abstract

The economic and ecological problems associated with fossil fuel have raised interest in biofuel research in recent times in different parts of the world. The use of Eucalyptus forest waste biomass with no appreciable value to industries or for food as alternative and cost effective feedstock for bioethanol production was evaluated in this study. E. camaldulensis biomass (bark and leaves) were pretreated separately with acid (2M H2SO4) and Microwave irradiation (250V, 50Hz) prior to fermentation with Bacillus subtilis and Escherichia coli isolated from surrounding soil. Higher yield of reducing sugar were obtained from bark (43 %) and leaves (38.5 %) pretreated by microwave irradiation as compared with acid treated plant biomass. Similarly, Bioethanol volume and concentration of 34.89 g/l and 0.51 % respectively were higher in Microwave irradiated bark of E. camaldulensis at 21 days of fermentation when E. coli and B. subtilis were used in synergy The least bioethanol volume yield of 18.79 g/l and concentration of 0.12 % when bark and leaves of E. camaldulensis were combined was obtained on day 7 of fermentation using E. coli. The study concludes that the amount of dried wastes generated (37.8 kg) from one average stand of Eucalyptus tree could yield significant volume (131,884.2 g/l) of bioethanol when B. subtilis and E. coli are used in synergy.

Published in International Journal of Sustainable and Green Energy (Volume 4, Issue 2)
DOI 10.11648/j.ijrse.20150402.13
Page(s) 40-46
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Eucalyptus, Biomass, Bioethanol, Fermentation

References
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    Usman Yahaya, Umar Yahaya Abdullahi, Denwe Samuel Dangmwan, Muhammad Muktar Namadi. (2015). Bioethanol Production from Eucalyptus camaldulensis Wood Waste Using Bacillus subtilis and Escherichia coli Isolated from Soil in Afaka Forest Reserve, Kaduna State Nigeria. International Journal of Sustainable and Green Energy, 4(2), 40-46. https://doi.org/10.11648/j.ijrse.20150402.13

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    ACS Style

    Usman Yahaya; Umar Yahaya Abdullahi; Denwe Samuel Dangmwan; Muhammad Muktar Namadi. Bioethanol Production from Eucalyptus camaldulensis Wood Waste Using Bacillus subtilis and Escherichia coli Isolated from Soil in Afaka Forest Reserve, Kaduna State Nigeria. Int. J. Sustain. Green Energy 2015, 4(2), 40-46. doi: 10.11648/j.ijrse.20150402.13

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    AMA Style

    Usman Yahaya, Umar Yahaya Abdullahi, Denwe Samuel Dangmwan, Muhammad Muktar Namadi. Bioethanol Production from Eucalyptus camaldulensis Wood Waste Using Bacillus subtilis and Escherichia coli Isolated from Soil in Afaka Forest Reserve, Kaduna State Nigeria. Int J Sustain Green Energy. 2015;4(2):40-46. doi: 10.11648/j.ijrse.20150402.13

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  • @article{10.11648/j.ijrse.20150402.13,
      author = {Usman Yahaya and Umar Yahaya Abdullahi and Denwe Samuel Dangmwan and Muhammad Muktar Namadi},
      title = {Bioethanol Production from Eucalyptus camaldulensis Wood Waste Using Bacillus subtilis and Escherichia coli Isolated from Soil in Afaka Forest Reserve, Kaduna State Nigeria},
      journal = {International Journal of Sustainable and Green Energy},
      volume = {4},
      number = {2},
      pages = {40-46},
      doi = {10.11648/j.ijrse.20150402.13},
      url = {https://doi.org/10.11648/j.ijrse.20150402.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijrse.20150402.13},
      abstract = {The economic and ecological problems associated with fossil fuel have raised interest in biofuel research in recent times in different parts of the world. The use of Eucalyptus forest waste biomass with no appreciable value to industries or for food as alternative and cost effective feedstock for bioethanol production was evaluated in this study. E. camaldulensis biomass (bark and leaves) were pretreated separately with acid (2M H2SO4) and Microwave irradiation (250V, 50Hz) prior to fermentation with Bacillus subtilis and Escherichia coli isolated from surrounding soil. Higher yield of reducing sugar were obtained from bark (43 %) and leaves (38.5 %) pretreated by microwave irradiation as compared with acid treated plant biomass. Similarly, Bioethanol volume and concentration of 34.89 g/l and 0.51 % respectively were higher in Microwave irradiated bark of E. camaldulensis at 21 days of fermentation when E. coli and B. subtilis were used in synergy The least bioethanol volume yield of 18.79 g/l and concentration of 0.12 % when bark and leaves of E. camaldulensis were combined was obtained on day 7 of fermentation using E. coli. The study concludes that the amount of dried wastes generated (37.8 kg) from one average stand of Eucalyptus tree could yield significant volume (131,884.2 g/l) of bioethanol when B. subtilis and E. coli are used in synergy.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Bioethanol Production from Eucalyptus camaldulensis Wood Waste Using Bacillus subtilis and Escherichia coli Isolated from Soil in Afaka Forest Reserve, Kaduna State Nigeria
    AU  - Usman Yahaya
    AU  - Umar Yahaya Abdullahi
    AU  - Denwe Samuel Dangmwan
    AU  - Muhammad Muktar Namadi
    Y1  - 2015/03/30
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    DO  - 10.11648/j.ijrse.20150402.13
    T2  - International Journal of Sustainable and Green Energy
    JF  - International Journal of Sustainable and Green Energy
    JO  - International Journal of Sustainable and Green Energy
    SP  - 40
    EP  - 46
    PB  - Science Publishing Group
    SN  - 2575-1549
    UR  - https://doi.org/10.11648/j.ijrse.20150402.13
    AB  - The economic and ecological problems associated with fossil fuel have raised interest in biofuel research in recent times in different parts of the world. The use of Eucalyptus forest waste biomass with no appreciable value to industries or for food as alternative and cost effective feedstock for bioethanol production was evaluated in this study. E. camaldulensis biomass (bark and leaves) were pretreated separately with acid (2M H2SO4) and Microwave irradiation (250V, 50Hz) prior to fermentation with Bacillus subtilis and Escherichia coli isolated from surrounding soil. Higher yield of reducing sugar were obtained from bark (43 %) and leaves (38.5 %) pretreated by microwave irradiation as compared with acid treated plant biomass. Similarly, Bioethanol volume and concentration of 34.89 g/l and 0.51 % respectively were higher in Microwave irradiated bark of E. camaldulensis at 21 days of fermentation when E. coli and B. subtilis were used in synergy The least bioethanol volume yield of 18.79 g/l and concentration of 0.12 % when bark and leaves of E. camaldulensis were combined was obtained on day 7 of fermentation using E. coli. The study concludes that the amount of dried wastes generated (37.8 kg) from one average stand of Eucalyptus tree could yield significant volume (131,884.2 g/l) of bioethanol when B. subtilis and E. coli are used in synergy.
    VL  - 4
    IS  - 2
    ER  - 

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Author Information
  • Forestry Research Institute of Nigeria, Trial Afforestation Research Station, Kaduna, Nigeria

  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

  • Department of Chemistry, Nigerian Defence Academy, Kaduna, Nigeria

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