Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids

Mehdi Ghasemi; Yemen Atakishiyeva

doi:doi:10.11648/j.ajls.s.2015030103.12

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Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids

Mehdi Ghasemi, Yemen Atakishiyeva

Published in American Journal of Life Sciences (Volume 3, Issue 1-3)

Received: 12 June 2014 Accepted: 19 April 2015 Published: 6 May 2015

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Abstract

Poly unsaturated fatty acids (PUFAs) participate in activities of all organisms from energy source to structural components. Disruption of metabolism results in diseases like atherosclerosis and obesity. In this work, optimal conditions to production of arachidonic, eicosapentaenoic and gamma- linolenic acids has been conducted in the oil degrading fungi-Cephalosporium humicola IE, Mucor globosus 11 and Pythium irregulare LX isolated from soils in Absheron peninsula, Azerbaijan. The fermentation process carried out at physicochemical conditions, carbon and nitrogen sources, temperature, pH, rate of inoculum, enzyme activity, exogenous fat and phosphate; as well as the stressful situations. Growth in crude oil as optimal carbon source showed that suitable nitrogen was peptone for lipid biosynthesis. In stable temperature the optimal lipid productionoccurred at 28°C, whereas in “temperature shifting technique”, greater yields occurred at 28°C for 5 days followed by at 15°C for 2 days. C/N ratio ranged from 4 to 64 and the optimal medium for production was composed of 2.0% glucose and 0.25% yeast extract, with addition of 0.1% KH2PO4at neutral pH with 3% inoculum. Although the activities of the enzymes varied among the fungi, but the developmental profiles for all enzymes were practically similar. Additive fat and phosphate accelerated growth and enhanced PUFA production. Data showed that the procedure of adaptation of fungi motivated the increase of the rate of membrane phospholipids with a high quantity of PUFAs. This research is considered to be object of PUFA production as food supplements.

Published in	American Journal of Life Sciences (Volume 3, Issue 1-3) This article belongs to the Special Issue Industrial Biotechnology
DOI	10.11648/j.ajls.s.2015030103.12
Page(s)	5-15
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Ascomycetes, Zygomycetes, Oomycetes, Lipid, SCO, PUFA, Crude Oil, Omega

References

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Mehdi Ghasemi, Yemen Atakishiyeva. (2015). Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids. American Journal of Life Sciences, 3(1-3), 5-15. https://doi.org/10.11648/j.ajls.s.2015030103.12

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ACS Style

Mehdi Ghasemi; Yemen Atakishiyeva. Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids. Am. J. Life Sci. 2015, 3(1-3), 5-15. doi: 10.11648/j.ajls.s.2015030103.12

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Mehdi Ghasemi, Yemen Atakishiyeva. Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids. Am J Life Sci. 2015;3(1-3):5-15. doi: 10.11648/j.ajls.s.2015030103.12

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@article{10.11648/j.ajls.s.2015030103.12,
  author = {Mehdi Ghasemi and Yemen Atakishiyeva},
  title = {Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {1-3},
  pages = {5-15},
  doi = {10.11648/j.ajls.s.2015030103.12},
  url = {https://doi.org/10.11648/j.ajls.s.2015030103.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2015030103.12},
  abstract = {Poly unsaturated fatty acids (PUFAs) participate in activities of all organisms from energy source to structural components. Disruption of metabolism results in diseases like atherosclerosis and obesity. In this work, optimal conditions to production of arachidonic, eicosapentaenoic and gamma- linolenic acids has been conducted in the oil degrading fungi-Cephalosporium humicola IE, Mucor globosus 11 and Pythium irregulare LX isolated from soils in Absheron peninsula, Azerbaijan. The fermentation process carried out at physicochemical conditions, carbon and nitrogen sources, temperature, pH, rate of inoculum, enzyme activity, exogenous fat and phosphate; as well as the stressful situations. Growth in crude oil as optimal carbon source showed that suitable nitrogen was peptone for lipid biosynthesis. In stable temperature the optimal lipid productionoccurred at 28°C, whereas in “temperature shifting technique”, greater yields occurred at 28°C for 5 days followed by at 15°C for 2 days. C/N ratio ranged from 4 to 64 and the optimal medium for production was composed of 2.0% glucose and 0.25% yeast extract, with addition of 0.1% KH2PO4at neutral pH with 3% inoculum. Although the activities of the enzymes varied among the fungi, but the developmental profiles for all enzymes were practically similar. Additive fat and phosphate accelerated growth and enhanced PUFA production. Data showed that the procedure of adaptation of fungi motivated the increase of the rate of membrane phospholipids with a high quantity of PUFAs. This research is considered to be object of PUFA production as food supplements.},
 year = {2015}
}

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TY  - JOUR
T1  - Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids
AU  - Mehdi Ghasemi
AU  - Yemen Atakishiyeva
Y1  - 2015/05/06
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.s.2015030103.12
DO  - 10.11648/j.ajls.s.2015030103.12
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 5
EP  - 15
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.s.2015030103.12
AB  - Poly unsaturated fatty acids (PUFAs) participate in activities of all organisms from energy source to structural components. Disruption of metabolism results in diseases like atherosclerosis and obesity. In this work, optimal conditions to production of arachidonic, eicosapentaenoic and gamma- linolenic acids has been conducted in the oil degrading fungi-Cephalosporium humicola IE, Mucor globosus 11 and Pythium irregulare LX isolated from soils in Absheron peninsula, Azerbaijan. The fermentation process carried out at physicochemical conditions, carbon and nitrogen sources, temperature, pH, rate of inoculum, enzyme activity, exogenous fat and phosphate; as well as the stressful situations. Growth in crude oil as optimal carbon source showed that suitable nitrogen was peptone for lipid biosynthesis. In stable temperature the optimal lipid productionoccurred at 28°C, whereas in “temperature shifting technique”, greater yields occurred at 28°C for 5 days followed by at 15°C for 2 days. C/N ratio ranged from 4 to 64 and the optimal medium for production was composed of 2.0% glucose and 0.25% yeast extract, with addition of 0.1% KH2PO4at neutral pH with 3% inoculum. Although the activities of the enzymes varied among the fungi, but the developmental profiles for all enzymes were practically similar. Additive fat and phosphate accelerated growth and enhanced PUFA production. Data showed that the procedure of adaptation of fungi motivated the increase of the rate of membrane phospholipids with a high quantity of PUFAs. This research is considered to be object of PUFA production as food supplements.
VL  - 3
IS  - 1-3
ER  -

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Author Information

Mehdi Ghasemi

Institute of microbiology, Azerbaijan National Academy of Sciences, Baku, Azerbaijan
Yemen Atakishiyeva

Institute of microbiology, Azerbaijan National Academy of Sciences, Baku, Azerbaijan

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Mehdi Ghasemi, Yemen Atakishiyeva. (2015). Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids. American Journal of Life Sciences, 3(1-3), 5-15. https://doi.org/10.11648/j.ajls.s.2015030103.12

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ACS Style

Mehdi Ghasemi; Yemen Atakishiyeva. Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids. Am. J. Life Sci. 2015, 3(1-3), 5-15. doi: 10.11648/j.ajls.s.2015030103.12

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Mehdi Ghasemi, Yemen Atakishiyeva. Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids. Am J Life Sci. 2015;3(1-3):5-15. doi: 10.11648/j.ajls.s.2015030103.12

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@article{10.11648/j.ajls.s.2015030103.12,
  author = {Mehdi Ghasemi and Yemen Atakishiyeva},
  title = {Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {1-3},
  pages = {5-15},
  doi = {10.11648/j.ajls.s.2015030103.12},
  url = {https://doi.org/10.11648/j.ajls.s.2015030103.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2015030103.12},
  abstract = {Poly unsaturated fatty acids (PUFAs) participate in activities of all organisms from energy source to structural components. Disruption of metabolism results in diseases like atherosclerosis and obesity. In this work, optimal conditions to production of arachidonic, eicosapentaenoic and gamma- linolenic acids has been conducted in the oil degrading fungi-Cephalosporium humicola IE, Mucor globosus 11 and Pythium irregulare LX isolated from soils in Absheron peninsula, Azerbaijan. The fermentation process carried out at physicochemical conditions, carbon and nitrogen sources, temperature, pH, rate of inoculum, enzyme activity, exogenous fat and phosphate; as well as the stressful situations. Growth in crude oil as optimal carbon source showed that suitable nitrogen was peptone for lipid biosynthesis. In stable temperature the optimal lipid productionoccurred at 28°C, whereas in “temperature shifting technique”, greater yields occurred at 28°C for 5 days followed by at 15°C for 2 days. C/N ratio ranged from 4 to 64 and the optimal medium for production was composed of 2.0% glucose and 0.25% yeast extract, with addition of 0.1% KH2PO4at neutral pH with 3% inoculum. Although the activities of the enzymes varied among the fungi, but the developmental profiles for all enzymes were practically similar. Additive fat and phosphate accelerated growth and enhanced PUFA production. Data showed that the procedure of adaptation of fungi motivated the increase of the rate of membrane phospholipids with a high quantity of PUFAs. This research is considered to be object of PUFA production as food supplements.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids
AU  - Mehdi Ghasemi
AU  - Yemen Atakishiyeva
Y1  - 2015/05/06
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.s.2015030103.12
DO  - 10.11648/j.ajls.s.2015030103.12
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 5
EP  - 15
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.s.2015030103.12
AB  - Poly unsaturated fatty acids (PUFAs) participate in activities of all organisms from energy source to structural components. Disruption of metabolism results in diseases like atherosclerosis and obesity. In this work, optimal conditions to production of arachidonic, eicosapentaenoic and gamma- linolenic acids has been conducted in the oil degrading fungi-Cephalosporium humicola IE, Mucor globosus 11 and Pythium irregulare LX isolated from soils in Absheron peninsula, Azerbaijan. The fermentation process carried out at physicochemical conditions, carbon and nitrogen sources, temperature, pH, rate of inoculum, enzyme activity, exogenous fat and phosphate; as well as the stressful situations. Growth in crude oil as optimal carbon source showed that suitable nitrogen was peptone for lipid biosynthesis. In stable temperature the optimal lipid productionoccurred at 28°C, whereas in “temperature shifting technique”, greater yields occurred at 28°C for 5 days followed by at 15°C for 2 days. C/N ratio ranged from 4 to 64 and the optimal medium for production was composed of 2.0% glucose and 0.25% yeast extract, with addition of 0.1% KH2PO4at neutral pH with 3% inoculum. Although the activities of the enzymes varied among the fungi, but the developmental profiles for all enzymes were practically similar. Additive fat and phosphate accelerated growth and enhanced PUFA production. Data showed that the procedure of adaptation of fungi motivated the increase of the rate of membrane phospholipids with a high quantity of PUFAs. This research is considered to be object of PUFA production as food supplements.
VL  - 3
IS  - 1-3
ER  -

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