Dendrobium jerdonianum Wight is an Epiphytic tufted orchid at higher elevations. The maximum percentage establishment of Flower stalk, leaf, node and internode explants was observed on MS, VW and KC Medium. However, MS media fortified with activated charcoal exhibited maximum multiplication rate for in vitro rooting. MS, VW and KC media supplemented with various concentrations of auxins and cytokinins were used in flower stalk, leaf, nodes and internodes for plantlet formation. In the evaluation of the media MS basal medium fortified with 0.5mg 2, 4, D-dichloro phenoxy acetic acid +5mg BAP + 50mlsuitable for flower stalk culture. KC medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid was found to be suitable for Leaf and internodes Culture. VW medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 3mg BAP + 50ml CM, was found to be suitable for nodal segments. The entire above medium used with different composition gives highest percentage of 80-95 percent results for plantlet formation. In vitro rooting was successful with MS medium supplemented with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 5mg IAA + 50ml CM and 500mg of activated charcoal. 90 days old in vitro plantlets were hardened and transferred to green house after ex vitro rooting technique. Significance of the present work is discussed here.
| Published in | American Journal of Life Sciences (Volume 4, Issue 3) |
| DOI | 10.11648/j.ajls.20160403.12 |
| Page(s) | 76-81 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Plantlet Formation, Explants, Dendrobium jerdonianum, Propagation, Establishment
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APA Style
Sr. Sagaya Mary B., Divakar K. M. (2016). In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants. American Journal of Life Sciences, 4(3), 76-81. https://doi.org/10.11648/j.ajls.20160403.12
ACS Style
Sr. Sagaya Mary B.; Divakar K. M. In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants. Am. J. Life Sci. 2016, 4(3), 76-81. doi: 10.11648/j.ajls.20160403.12
AMA Style
Sr. Sagaya Mary B., Divakar K. M. In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants. Am J Life Sci. 2016;4(3):76-81. doi: 10.11648/j.ajls.20160403.12
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Download@article{10.11648/j.ajls.20160403.12,
author = {Sr. Sagaya Mary B. and Divakar K. M.},
title = {In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants},
journal = {American Journal of Life Sciences},
volume = {4},
number = {3},
pages = {76-81},
doi = {10.11648/j.ajls.20160403.12},
url = {https://doi.org/10.11648/j.ajls.20160403.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160403.12},
abstract = {Dendrobium jerdonianum Wight is an Epiphytic tufted orchid at higher elevations. The maximum percentage establishment of Flower stalk, leaf, node and internode explants was observed on MS, VW and KC Medium. However, MS media fortified with activated charcoal exhibited maximum multiplication rate for in vitro rooting. MS, VW and KC media supplemented with various concentrations of auxins and cytokinins were used in flower stalk, leaf, nodes and internodes for plantlet formation. In the evaluation of the media MS basal medium fortified with 0.5mg 2, 4, D-dichloro phenoxy acetic acid +5mg BAP + 50mlsuitable for flower stalk culture. KC medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid was found to be suitable for Leaf and internodes Culture. VW medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 3mg BAP + 50ml CM, was found to be suitable for nodal segments. The entire above medium used with different composition gives highest percentage of 80-95 percent results for plantlet formation. In vitro rooting was successful with MS medium supplemented with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 5mg IAA + 50ml CM and 500mg of activated charcoal. 90 days old in vitro plantlets were hardened and transferred to green house after ex vitro rooting technique. Significance of the present work is discussed here.},
year = {2016}
}
TY - JOUR T1 - In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants AU - Sr. Sagaya Mary B. AU - Divakar K. M. Y1 - 2016/06/17 PY - 2016 N1 - https://doi.org/10.11648/j.ajls.20160403.12 DO - 10.11648/j.ajls.20160403.12 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 76 EP - 81 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20160403.12 AB - Dendrobium jerdonianum Wight is an Epiphytic tufted orchid at higher elevations. The maximum percentage establishment of Flower stalk, leaf, node and internode explants was observed on MS, VW and KC Medium. However, MS media fortified with activated charcoal exhibited maximum multiplication rate for in vitro rooting. MS, VW and KC media supplemented with various concentrations of auxins and cytokinins were used in flower stalk, leaf, nodes and internodes for plantlet formation. In the evaluation of the media MS basal medium fortified with 0.5mg 2, 4, D-dichloro phenoxy acetic acid +5mg BAP + 50mlsuitable for flower stalk culture. KC medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid was found to be suitable for Leaf and internodes Culture. VW medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 3mg BAP + 50ml CM, was found to be suitable for nodal segments. The entire above medium used with different composition gives highest percentage of 80-95 percent results for plantlet formation. In vitro rooting was successful with MS medium supplemented with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 5mg IAA + 50ml CM and 500mg of activated charcoal. 90 days old in vitro plantlets were hardened and transferred to green house after ex vitro rooting technique. Significance of the present work is discussed here. VL - 4 IS - 3 ER -
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