Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections

Hatil Hashim Elkamali; Sana Eltayeb Mahjoob

doi:doi:10.11648/j.ajls.20150303.25

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Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections

Hatil Hashim Elkamali, Sana Eltayeb Mahjoob

Published in American Journal of Life Sciences (Volume 3, Issue 3)

Received: 8 May 2015 Accepted: 1 June 2015 Published: 14 June 2015

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Abstract

Punica granatum, Citrullus colocynthes, Curcuma long, Albizia lebbeckandAzardirachta indicaare used in traditional medical practice in Sudan for the treatment of gastrointestinal tract infections. The methanol and aqueous extracts from different plant parts (Peel of fruits, rhizome, fruits, leaves, and Gum) respectively at a concentration of 100 mg/ml, were evaluated against 20 clinical isolates (2 were Salmonella typhi,5 Proteus mirabilis,4 Escherichia coli,5 Pseudomonas aeruginosa,3 Staphylococcus aureus, one was Salmonella para typhi B) and 5 standard bacterial strains (Staphylococcusaureus ATCC 25923), Bacillus subtilis (NCTC 8236), Escherichia coli (ATCC 25922), Salmonella typhi (ATCC1319106) and Klebsiella pneumoniae (ATCC 35657) were tested for their antibacterial properties using the Agar Diffusion Technique in vitro. Of all plants methanolic and aqueous extracts of Punica granatum were the most active with clinical isolates and standard bacterial strains showed relatively high antibacterial activity against most of the tested microorganisms with the diameter of inhibition zones ranging between 14 and 24 mm, whereas the methanolic extract of Curcuma long showed high antibacterial activity againstProteus mirabilis clinical isolate (1Z = 20 mm). Most susceptible Gram-negative clinical Isolates bacteria were Escherichia coli and Proteusmirabilis. Most susceptible Gram negative standard bacteria were Bacillus subtilis (NCTC 8236) and Escherichia coli (ATCC 25922) and least susceptible Gram negative bacterium was Klebsiella pneumoniae (ATCC 35657).In Gram positive standard bacteria, most susceptible was S.aureus (ATCC 25923).Antibiotics was used as standards drug for antibacterial assay. The results obtained appeared to confirm the antibacterial potential of the plants investigated, and their usefulness in the treatment of gastrointestinal tract infections.

Published in	American Journal of Life Sciences (Volume 3, Issue 3)
DOI	10.11648/j.ajls.20150303.25
Page(s)	230-237
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Antibacterialactivity, Medicinal Plants, Clinical Isolates and Standard Pathogenic Bacteria

References

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APA Style

Hatil Hashim Elkamali, Sana Eltayeb Mahjoob. (2015). Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections. American Journal of Life Sciences, 3(3), 230-237. https://doi.org/10.11648/j.ajls.20150303.25

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ACS Style

Hatil Hashim Elkamali; Sana Eltayeb Mahjoob. Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections. Am. J. Life Sci. 2015, 3(3), 230-237. doi: 10.11648/j.ajls.20150303.25

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AMA Style

Hatil Hashim Elkamali, Sana Eltayeb Mahjoob. Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections. Am J Life Sci. 2015;3(3):230-237. doi: 10.11648/j.ajls.20150303.25

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@article{10.11648/j.ajls.20150303.25,
  author = {Hatil Hashim Elkamali and Sana Eltayeb Mahjoob},
  title = {Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {3},
  pages = {230-237},
  doi = {10.11648/j.ajls.20150303.25},
  url = {https://doi.org/10.11648/j.ajls.20150303.25},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150303.25},
  abstract = {Punica granatum, Citrullus colocynthes, Curcuma long, Albizia lebbeckandAzardirachta indicaare used in traditional medical practice in Sudan for the treatment of gastrointestinal tract infections. The methanol and aqueous extracts from different plant parts (Peel of fruits, rhizome, fruits, leaves, and Gum) respectively at a concentration of 100 mg/ml, were evaluated against 20 clinical isolates (2 were Salmonella typhi,5 Proteus mirabilis,4 Escherichia coli,5 Pseudomonas aeruginosa,3 Staphylococcus aureus, one was Salmonella para typhi B) and 5 standard bacterial strains (Staphylococcusaureus ATCC 25923), Bacillus subtilis (NCTC 8236), Escherichia coli (ATCC 25922), Salmonella typhi (ATCC1319106) and Klebsiella pneumoniae (ATCC 35657) were tested for their antibacterial properties using the Agar Diffusion Technique in vitro. Of all plants methanolic and aqueous extracts of Punica granatum were the most active with clinical isolates and standard bacterial strains showed relatively high antibacterial activity against most of the tested microorganisms with the diameter of inhibition zones ranging between 14 and 24 mm, whereas the methanolic extract of Curcuma long showed high antibacterial activity againstProteus mirabilis clinical isolate (1Z = 20 mm). Most susceptible Gram-negative clinical Isolates bacteria were Escherichia coli and Proteusmirabilis. Most susceptible Gram negative standard bacteria were Bacillus subtilis (NCTC 8236) and Escherichia coli (ATCC 25922) and least susceptible Gram negative bacterium was Klebsiella pneumoniae (ATCC 35657).In Gram positive standard bacteria, most susceptible was S.aureus (ATCC 25923).Antibiotics was used as standards drug for antibacterial assay. The results obtained appeared to confirm the antibacterial potential of the plants investigated, and their usefulness in the treatment of gastrointestinal tract infections.},
 year = {2015}
}

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TY  - JOUR
T1  - Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections
AU  - Hatil Hashim Elkamali
AU  - Sana Eltayeb Mahjoob
Y1  - 2015/06/14
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.20150303.25
DO  - 10.11648/j.ajls.20150303.25
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 230
EP  - 237
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20150303.25
AB  - Punica granatum, Citrullus colocynthes, Curcuma long, Albizia lebbeckandAzardirachta indicaare used in traditional medical practice in Sudan for the treatment of gastrointestinal tract infections. The methanol and aqueous extracts from different plant parts (Peel of fruits, rhizome, fruits, leaves, and Gum) respectively at a concentration of 100 mg/ml, were evaluated against 20 clinical isolates (2 were Salmonella typhi,5 Proteus mirabilis,4 Escherichia coli,5 Pseudomonas aeruginosa,3 Staphylococcus aureus, one was Salmonella para typhi B) and 5 standard bacterial strains (Staphylococcusaureus ATCC 25923), Bacillus subtilis (NCTC 8236), Escherichia coli (ATCC 25922), Salmonella typhi (ATCC1319106) and Klebsiella pneumoniae (ATCC 35657) were tested for their antibacterial properties using the Agar Diffusion Technique in vitro. Of all plants methanolic and aqueous extracts of Punica granatum were the most active with clinical isolates and standard bacterial strains showed relatively high antibacterial activity against most of the tested microorganisms with the diameter of inhibition zones ranging between 14 and 24 mm, whereas the methanolic extract of Curcuma long showed high antibacterial activity againstProteus mirabilis clinical isolate (1Z = 20 mm). Most susceptible Gram-negative clinical Isolates bacteria were Escherichia coli and Proteusmirabilis. Most susceptible Gram negative standard bacteria were Bacillus subtilis (NCTC 8236) and Escherichia coli (ATCC 25922) and least susceptible Gram negative bacterium was Klebsiella pneumoniae (ATCC 35657).In Gram positive standard bacteria, most susceptible was S.aureus (ATCC 25923).Antibiotics was used as standards drug for antibacterial assay. The results obtained appeared to confirm the antibacterial potential of the plants investigated, and their usefulness in the treatment of gastrointestinal tract infections.
VL  - 3
IS  - 3
ER  -

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Author Information

Hatil Hashim Elkamali

Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan
Sana Eltayeb Mahjoob

Department of Biochemistry, Faculty of Medical Laboratories Science, Omdurman Islamic University, Omdurman, Sudan

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Hatil Hashim Elkamali, Sana Eltayeb Mahjoob. (2015). Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections. American Journal of Life Sciences, 3(3), 230-237. https://doi.org/10.11648/j.ajls.20150303.25

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ACS Style

Hatil Hashim Elkamali; Sana Eltayeb Mahjoob. Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections. Am. J. Life Sci. 2015, 3(3), 230-237. doi: 10.11648/j.ajls.20150303.25

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AMA Style

Hatil Hashim Elkamali, Sana Eltayeb Mahjoob. Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections. Am J Life Sci. 2015;3(3):230-237. doi: 10.11648/j.ajls.20150303.25

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@article{10.11648/j.ajls.20150303.25,
  author = {Hatil Hashim Elkamali and Sana Eltayeb Mahjoob},
  title = {Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {3},
  pages = {230-237},
  doi = {10.11648/j.ajls.20150303.25},
  url = {https://doi.org/10.11648/j.ajls.20150303.25},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150303.25},
  abstract = {Punica granatum, Citrullus colocynthes, Curcuma long, Albizia lebbeckandAzardirachta indicaare used in traditional medical practice in Sudan for the treatment of gastrointestinal tract infections. The methanol and aqueous extracts from different plant parts (Peel of fruits, rhizome, fruits, leaves, and Gum) respectively at a concentration of 100 mg/ml, were evaluated against 20 clinical isolates (2 were Salmonella typhi,5 Proteus mirabilis,4 Escherichia coli,5 Pseudomonas aeruginosa,3 Staphylococcus aureus, one was Salmonella para typhi B) and 5 standard bacterial strains (Staphylococcusaureus ATCC 25923), Bacillus subtilis (NCTC 8236), Escherichia coli (ATCC 25922), Salmonella typhi (ATCC1319106) and Klebsiella pneumoniae (ATCC 35657) were tested for their antibacterial properties using the Agar Diffusion Technique in vitro. Of all plants methanolic and aqueous extracts of Punica granatum were the most active with clinical isolates and standard bacterial strains showed relatively high antibacterial activity against most of the tested microorganisms with the diameter of inhibition zones ranging between 14 and 24 mm, whereas the methanolic extract of Curcuma long showed high antibacterial activity againstProteus mirabilis clinical isolate (1Z = 20 mm). Most susceptible Gram-negative clinical Isolates bacteria were Escherichia coli and Proteusmirabilis. Most susceptible Gram negative standard bacteria were Bacillus subtilis (NCTC 8236) and Escherichia coli (ATCC 25922) and least susceptible Gram negative bacterium was Klebsiella pneumoniae (ATCC 35657).In Gram positive standard bacteria, most susceptible was S.aureus (ATCC 25923).Antibiotics was used as standards drug for antibacterial assay. The results obtained appeared to confirm the antibacterial potential of the plants investigated, and their usefulness in the treatment of gastrointestinal tract infections.},
 year = {2015}
}

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TY  - JOUR
T1  - Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections
AU  - Hatil Hashim Elkamali
AU  - Sana Eltayeb Mahjoob
Y1  - 2015/06/14
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.20150303.25
DO  - 10.11648/j.ajls.20150303.25
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 230
EP  - 237
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20150303.25
AB  - Punica granatum, Citrullus colocynthes, Curcuma long, Albizia lebbeckandAzardirachta indicaare used in traditional medical practice in Sudan for the treatment of gastrointestinal tract infections. The methanol and aqueous extracts from different plant parts (Peel of fruits, rhizome, fruits, leaves, and Gum) respectively at a concentration of 100 mg/ml, were evaluated against 20 clinical isolates (2 were Salmonella typhi,5 Proteus mirabilis,4 Escherichia coli,5 Pseudomonas aeruginosa,3 Staphylococcus aureus, one was Salmonella para typhi B) and 5 standard bacterial strains (Staphylococcusaureus ATCC 25923), Bacillus subtilis (NCTC 8236), Escherichia coli (ATCC 25922), Salmonella typhi (ATCC1319106) and Klebsiella pneumoniae (ATCC 35657) were tested for their antibacterial properties using the Agar Diffusion Technique in vitro. Of all plants methanolic and aqueous extracts of Punica granatum were the most active with clinical isolates and standard bacterial strains showed relatively high antibacterial activity against most of the tested microorganisms with the diameter of inhibition zones ranging between 14 and 24 mm, whereas the methanolic extract of Curcuma long showed high antibacterial activity againstProteus mirabilis clinical isolate (1Z = 20 mm). Most susceptible Gram-negative clinical Isolates bacteria were Escherichia coli and Proteusmirabilis. Most susceptible Gram negative standard bacteria were Bacillus subtilis (NCTC 8236) and Escherichia coli (ATCC 25922) and least susceptible Gram negative bacterium was Klebsiella pneumoniae (ATCC 35657).In Gram positive standard bacteria, most susceptible was S.aureus (ATCC 25923).Antibiotics was used as standards drug for antibacterial assay. The results obtained appeared to confirm the antibacterial potential of the plants investigated, and their usefulness in the treatment of gastrointestinal tract infections.
VL  - 3
IS  - 3
ER  -

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