Effect of Aqueous Leaf Extract of Annona senegalensis on Selected Testicular Function Indices of Wistar Rats

Charles Obiora Nwonuma; Emenike Onyebum Irokanulo; Adeyemi Eunice Jolaiya; Ayokanmi Ore

doi:doi:10.11648/j.ajls.20150303.21

| Peer-Reviewed

Effect of Aqueous Leaf Extract of Annona senegalensis on Selected Testicular Function Indices of Wistar Rats

Charles Obiora Nwonuma, Emenike Onyebum Irokanulo, Adeyemi Eunice Jolaiya, Ayokanmi Ore

Published in American Journal of Life Sciences (Volume 3, Issue 3)

Received: 9 May 2015 Accepted: 15 May 2015 Published: 27 May 2015

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

This study evaluated the effect of Annona senegalensis on selected biochemical and testicular function indices in rats. Twenty Wistar rats (125 – 127 g) were randomly assigned into four experimental groups: A, B, C and D, (n = 5/ group). Animals in group A served as control and was administered distilled water, while animals in groups B, C, and D were given 100 mg/kg body weight (bw), 200 mg/kg bw and 300 mg/kg bw of aqueous leaf extract of Annona senegalensis (ALEAS) respectively by oral gavage for 21 days. Data revealed a significant decrease (P < 0.05) in the testis-body weight ratio and total protein content in the testes of rats administered ALEAS compared to the control. There was also a significant increase (P < 0.05) in testicular cholesterol, glycogen, malondialdhyde (MDA) and catalase (CAT) activity. Additionally, histopathological studies revealed a marked degeneration of seminiferous tubules. Overall, data from the present study suggest that aqueous leaf extract of Annona senegalensis may possess the potential to adversely affect testicular function in rat.

Published in	American Journal of Life Sciences (Volume 3, Issue 3)
DOI	10.11648/j.ajls.20150303.21
Page(s)	203-212
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Annona senegalensis, Testes, Testicular Function, Histopathology, Rat

References

[1]	C. O. Okoli, C. A. Onyeto, B. P. Akpa, A. C. Ezike, P. A. Akah and T. C. Okoye, “Neuropharmacological evaluation of Annona senegalensis leaves,” Afr. J. Biotechnol., 9, (49), 8435-8444, 2010.
[2]	H. M. Burkill, “The Useful Plants of West Africa,” Royal Botanical Gardens, Kew, pp. 103–105, 1985.
[3]	D. N. Muanza, B. W. Kim, K. L. Euler and L. Williams, “Antibacterial and antifungal activities of nine medicinal plants from Zaire,” Int’l J. Pharmacog. 32, 337–345, 1994.
[4]	J. I. Durodola, “Viability and transplanability of developed tumour cells treated in vitro with antitumour agent C/M2 isolated from herbal cancer remedy of Annona senegalensis,” Planta Medica, 28, 359, 1975.
[5]	M. O. Fatope, H. Ibrahim, and Y. Takeda, “Screening of higher plants reputed as pesticides using the brine shrimp lethality assay,” Int’l J. Pharmacog. 31, 250–254, 1993.
[6]	T. O. E. Ekpendu, O. D. Obande, P. O. Anyogo and A. D. Attah, ”Nigerian ethnomedicine and medicinal plant flora – the Benue experience part 1”. J. Pharm. Res. Dev., 3, 37–46, 1998.
[7]	A. C. Kudi and S. H. Myint, “Antiviral activity of some Nigerian medicinal plant extracts,” J. Ethnopharmacol, 68, 289–294, 1999.
[8]	J. Audu, “Medicinal herbs and their uses in Bauchi State,” The Nig Field, 54, 157–168, 1989.
[9]	J. M. Dalziel, “The useful plants of West Tropical Africa,” Crownoverseas agents for the colonies,” London. pp. 2–3, 1937.
[10]	R. B. F. Langason, D. N. Akunyili, and P. I. Akubue, “A Preliminary study of the gastrointestinal effects of some Nigerian medicinal plants,” Fitoterapia, 65, 235–240, 1994.
[11]	A. G. Igweii and A. O. Onabanjo, “Chemotherapeutic effects of Annona senegalensisin Trypanasoma brucei brucei,” Ann Trop. Med. Parasitol. 83, 527–534, 1989.
[12]	E. A. Sofowora and C. O. Adewunmi, “Preliminary screening of some plant extracts for molluscidal activity,” Planta Medica 39, 57–65, 1980.
[13]	M. Jacobson, R. E. Redfern and G. D. Mills. Naturally occurring insect growth regulators 11. Screening of insect and plant extracts as juvenile hormone mimics. Lloydia 38, 455–472, 1975.
[14]	A. A. Mustapha, “Annona senegalensis Persoon: A Multipurpose shrub, its Phytotherapic, Phytopharmacological and Phytomedicinal Uses,” International Journal of Science and Technology, 2(12), 862-865, 2013.
[15]	C. B. Alawa, A. M. Adamu, J. O. Gefu, O. J. Ajanusi, P. A. Abdu, N. P. Chiezey, J. N. Alawa and D. D. Bowman, “In vitro screening of two Nigerian medicinal plants (Vernonia amygdalina and Annona senegalensis) for anthelmintic activity,” Vet Parasitol. 113(1), 73-81, 2003.
[16]	National Research Council. Guide for the Care and Use of Laboratory Animals, 8th ed.; The National Academies Press: Washington, DC, USA, 2011.
[17]	A. G. Gornall, C. J. Bardwawill, and M. M. David, “Determination of serum proteins by means of the biuret reaction,” The Journal of biological chemistry, 177(2), 751–766, 1949.
[18]	K. A. Sinha, “Colorimetric assay of catalase. Annual Biochemistry, 47, 389-394, 1972.
[19]	H. P. Misra and I. Fridovich, “The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase,” J.Biol Chem., 247 (10), 3170 – 3175, 1972.
[20]	K. Satoh, “Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method,” Clinica Chimica Acta, 90, 37-43, 1978.
[21]	A. Kemp, J. M. Adrienne and K. V. Heijningen, “A colorimetric micro-method for the determination of glycogen in tissues,” Biochem. J., 56, 646-648, 1954.
[22]	D. S. Fredrickson, R. I. Levy and R. S. Lees, “Fat transport in lipoproteins – an integrated approach to mechanisms and disorders,” New Engl. J. Med., 276, 215– 225, 1967.
[23]	P. J. Wright, P. D. Leathwood and D. T. Plummer, “Enzymes in rat urine: acid-phosphatase,” Enzymologia, 42(6), 459-68, 1972.
[24]	P. J. Wright, P. D. Leathwood and D. T. Plummer, “Enzyme in rat urine Alkaline phosphatase,” Enzymologia 42, 317-327, 1972.
[25]	O. S. Adeyemi and M. A. Akanji, “psidium guava leaf extract; effects on rats’ serum homeostasis and tissue morphology,” Comp. Clin. Pathol. 2010.
[26]	M. T. Yakubu and Q. O. Nurudeen, “Effects of aqueous extract of Cnestis ferruginea (Vahl ex De Cantolle) root on paroxetine-induced sexual dysfunction in male rats,” Asian Pac. J. Reprod., 1 (2):111–116, 2012.
[27]	S. C. Joshi, N. Gulati and A. Gajraj, “Evaluation of toxic impacts of mancozeb on testis in rats,” Asian J. Exp. Sci., 19(1), 73-83, 2005.
[28]	R. L. Ksheerasagar, and B. B. Kaliwal, “Temporal effects of mancozeb on testes,accessory reproductive organs and biochemical constituents in albino mice,” Envirot. Toxicol. Pharmacol. 15: 9-17, 2003.
[29]	R. S. Gupta, R. Sharma, A. Sharma, A. K. Bhatnager, M. P. Dobhal, Y. C. Joshi and M. C. Sharma, “Effect of Alstonia scholaris bark extract on testicular function of wistar rats,” Asian J. Androl., 4, 175–178, 2002.
[30]	P. Kamatchouing, Y. M. Fandio, T. Dimo, H. B. Jatsa, “Evaluation of androgenic activity of Zingiber officinale and Pentadiplandra brazzeana in male rats,” Asian J. Androl. 4, 299–301, 2002.
[31]	P. Watcho, P. Kamtchouing, S. D. Sokeng, P. F. Moundipa, J. Tantchou, J. L. Essame and N. Koueta, “Androgenic effect of Mondia whitei roots in male rats,” Asian J. Androl., 6, 269–272, 2004.
[32]	N. Sofikitis and I. Miyagawa, “Secretary dysfunction of the male accessory genital gland due to prostate infections and fertility: A selected review of the literature,” Jpn. Fertil. Steril. 36, 690-699, 1991.
[33]	M. A. Adaikpoh and F. O. Obi, “Prevention of cadmium-induced alteration in rat testes and prostate lipid patterns by –tocopherol,” Afr. J. Biochem. Res., 3 (10), 321-325, 2009.
[34]	J. B. Kerr, R. A. Mayberry and D. C. Irby, “Morphometric studies on lipid inclusions in Sertoli cells during the spermatogenic cycle in the rat,” Cell Tissue Res., 236, 699-709, 1984.
[35]	Y. Shima, K. Miyabayashi, S. Haraguchi, T. Arakawa, H. Otake, T. Baba, S. Matsuzaki, Y. Shishido, H. Akiyama, T. Tachibana, K. Tsutsui, K. Morohashi, “Contribution of Leydig and Sertoli cells to testosterone production in mouse fetal testes,” Mol. Endocrinol. 27 (1), 63-73, 2013.
[36]	I. S. Farooqi and S. O. Rahilly, “Monogenic Human Obesity Syndromes,”. Recent Prog. Horm. Res., 59, 409-424, 2004.
[37]	F. Bustos-Obregon and J. R. Gonzalez, “Melatonin as protective agent for the cytotoxic effects of diazinon in the spermatogenesis in the earthworm Eisenia foetida,” Ital. J. Anat. Embryol., 110 (2) 159-165, 2005.
[38]	C. O. Ikediobi, V. L. Badisa, L. T. Ayuk-Takem, L. M. Latinwo and J. West, “Response of antioxidant enzymes and redox metabolites to cadmium-induced oxidative stress in CRL-1439 normal bliver cells,” Int. J. Mol. Med., 14, 87–92, 2004.
[39]	J. D. Eneman, R. J. Potis, M. Osier, G. S. Shukla, C. H. B. Lee and J. F. Chin, “Suppressed oxidant-induced apoptosis in cadmium adopted alveolar epithelial cells and its potential involvement in cadmium carcinogenesis,” Toxicol., 147, 215–228, 2000.
[40]	Z. Spolarics and J. X. Wu, “Role of glutathione and catalase in H2O2 detoxification in LPS-activated hepatic endothelial and Kupffer cells,” Gastroint. Liver Physiol., 273, G1304, 1997.
[41]	V. Ramalingam and V. Vimaladevi,. “Effect of mercuric chloride on membrane-bound enzymes in rat testis,” Asian J. Androl., 4, 309–311, 2002.
[42]	S. Breton, P. T. S. Smith, B. Lui and D. Brown, “Acidification of the male reproductive tract by a proton pumping (H+)-ATPase,” Natural Medicine, 2, 470–472, 1996.
[43]	S. M. Cherry, P. A. Hunt and T. J. Hassold, “Cisplatin distrupts mammalian spermatogenesis, but does not affect recombination or chromosome segregation,” Mutat. Res., 564, 115-128, 2004.

Cite This Article

Plain Text BibTeX RIS

APA Style

Charles Obiora Nwonuma, Emenike Onyebum Irokanulo, Adeyemi Eunice Jolaiya, Ayokanmi Ore. (2015). Effect of Aqueous Leaf Extract of Annona senegalensis on Selected Testicular Function Indices of Wistar Rats. American Journal of Life Sciences, 3(3), 203-212. https://doi.org/10.11648/j.ajls.20150303.21

Copy | Download

ACS Style

Charles Obiora Nwonuma; Emenike Onyebum Irokanulo; Adeyemi Eunice Jolaiya; Ayokanmi Ore. Effect of Aqueous Leaf Extract of Annona senegalensis on Selected Testicular Function Indices of Wistar Rats. Am. J. Life Sci. 2015, 3(3), 203-212. doi: 10.11648/j.ajls.20150303.21

Copy | Download

AMA Style

Charles Obiora Nwonuma, Emenike Onyebum Irokanulo, Adeyemi Eunice Jolaiya, Ayokanmi Ore. Effect of Aqueous Leaf Extract of Annona senegalensis on Selected Testicular Function Indices of Wistar Rats. Am J Life Sci. 2015;3(3):203-212. doi: 10.11648/j.ajls.20150303.21

Copy | Download

@article{10.11648/j.ajls.20150303.21,
  author = {Charles Obiora Nwonuma and Emenike Onyebum Irokanulo and Adeyemi Eunice Jolaiya and Ayokanmi Ore},
  title = {Effect of Aqueous Leaf Extract of Annona senegalensis on Selected Testicular Function Indices of Wistar Rats},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {3},
  pages = {203-212},
  doi = {10.11648/j.ajls.20150303.21},
  url = {https://doi.org/10.11648/j.ajls.20150303.21},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150303.21},
  abstract = {This study evaluated the effect of Annona senegalensis on selected biochemical and testicular function indices in rats. Twenty Wistar rats (125 – 127 g) were randomly assigned into four experimental groups: A, B, C and D, (n = 5/ group). Animals in group A served as control and was administered distilled water, while animals in groups B, C, and D were given 100 mg/kg body weight (bw), 200 mg/kg bw and 300 mg/kg bw of aqueous leaf extract of Annona senegalensis (ALEAS) respectively by oral gavage for 21 days. Data revealed a significant decrease (P < 0.05) in the testis-body weight ratio and total protein content in the testes of rats administered ALEAS compared to the control. There was also a significant increase (P < 0.05) in testicular cholesterol, glycogen, malondialdhyde (MDA) and catalase (CAT) activity. Additionally, histopathological studies revealed a marked degeneration of seminiferous tubules. Overall, data from the present study suggest that aqueous leaf extract of Annona senegalensis may possess the potential to adversely affect testicular function in rat.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Effect of Aqueous Leaf Extract of Annona senegalensis on Selected Testicular Function Indices of Wistar Rats
AU  - Charles Obiora Nwonuma
AU  - Emenike Onyebum Irokanulo
AU  - Adeyemi Eunice Jolaiya
AU  - Ayokanmi Ore
Y1  - 2015/05/27
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.20150303.21
DO  - 10.11648/j.ajls.20150303.21
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 203
EP  - 212
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20150303.21
AB  - This study evaluated the effect of Annona senegalensis on selected biochemical and testicular function indices in rats. Twenty Wistar rats (125 – 127 g) were randomly assigned into four experimental groups: A, B, C and D, (n = 5/ group). Animals in group A served as control and was administered distilled water, while animals in groups B, C, and D were given 100 mg/kg body weight (bw), 200 mg/kg bw and 300 mg/kg bw of aqueous leaf extract of Annona senegalensis (ALEAS) respectively by oral gavage for 21 days. Data revealed a significant decrease (P < 0.05) in the testis-body weight ratio and total protein content in the testes of rats administered ALEAS compared to the control. There was also a significant increase (P < 0.05) in testicular cholesterol, glycogen, malondialdhyde (MDA) and catalase (CAT) activity. Additionally, histopathological studies revealed a marked degeneration of seminiferous tubules. Overall, data from the present study suggest that aqueous leaf extract of Annona senegalensis may possess the potential to adversely affect testicular function in rat.
VL  - 3
IS  - 3
ER  -

Copy | Download

Author Information

Charles Obiora Nwonuma

Biochemistry Unit, Department of Biological Sciences, College of Science and Engineering, Landmark University, Omuaran Kwara State, Nigeria
Emenike Onyebum Irokanulo

Biochemistry Unit, Department of Biological Sciences, College of Science and Engineering, Landmark University, Omuaran Kwara State, Nigeria
Adeyemi Eunice Jolaiya

Biochemistry Unit, Department of Biological Sciences, College of Science and Engineering, Landmark University, Omuaran Kwara State, Nigeria
Ayokanmi Ore

Biochemistry Unit, Department of Chemical Sciences, Faculty of Natural Sciences, Ajayi Crowther University, Oyo, Oyo State, Nigeria

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Charles Obiora Nwonuma, Emenike Onyebum Irokanulo, Adeyemi Eunice Jolaiya, Ayokanmi Ore. (2015). Effect of Aqueous Leaf Extract of Annona senegalensis on Selected Testicular Function Indices of Wistar Rats. American Journal of Life Sciences, 3(3), 203-212. https://doi.org/10.11648/j.ajls.20150303.21

Copy | Download

ACS Style

Charles Obiora Nwonuma; Emenike Onyebum Irokanulo; Adeyemi Eunice Jolaiya; Ayokanmi Ore. Effect of Aqueous Leaf Extract of Annona senegalensis on Selected Testicular Function Indices of Wistar Rats. Am. J. Life Sci. 2015, 3(3), 203-212. doi: 10.11648/j.ajls.20150303.21

Copy | Download

AMA Style

Charles Obiora Nwonuma, Emenike Onyebum Irokanulo, Adeyemi Eunice Jolaiya, Ayokanmi Ore. Effect of Aqueous Leaf Extract of Annona senegalensis on Selected Testicular Function Indices of Wistar Rats. Am J Life Sci. 2015;3(3):203-212. doi: 10.11648/j.ajls.20150303.21

Copy | Download

@article{10.11648/j.ajls.20150303.21,
  author = {Charles Obiora Nwonuma and Emenike Onyebum Irokanulo and Adeyemi Eunice Jolaiya and Ayokanmi Ore},
  title = {Effect of Aqueous Leaf Extract of Annona senegalensis on Selected Testicular Function Indices of Wistar Rats},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {3},
  pages = {203-212},
  doi = {10.11648/j.ajls.20150303.21},
  url = {https://doi.org/10.11648/j.ajls.20150303.21},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150303.21},
  abstract = {This study evaluated the effect of Annona senegalensis on selected biochemical and testicular function indices in rats. Twenty Wistar rats (125 – 127 g) were randomly assigned into four experimental groups: A, B, C and D, (n = 5/ group). Animals in group A served as control and was administered distilled water, while animals in groups B, C, and D were given 100 mg/kg body weight (bw), 200 mg/kg bw and 300 mg/kg bw of aqueous leaf extract of Annona senegalensis (ALEAS) respectively by oral gavage for 21 days. Data revealed a significant decrease (P < 0.05) in the testis-body weight ratio and total protein content in the testes of rats administered ALEAS compared to the control. There was also a significant increase (P < 0.05) in testicular cholesterol, glycogen, malondialdhyde (MDA) and catalase (CAT) activity. Additionally, histopathological studies revealed a marked degeneration of seminiferous tubules. Overall, data from the present study suggest that aqueous leaf extract of Annona senegalensis may possess the potential to adversely affect testicular function in rat.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Effect of Aqueous Leaf Extract of Annona senegalensis on Selected Testicular Function Indices of Wistar Rats
AU  - Charles Obiora Nwonuma
AU  - Emenike Onyebum Irokanulo
AU  - Adeyemi Eunice Jolaiya
AU  - Ayokanmi Ore
Y1  - 2015/05/27
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.20150303.21
DO  - 10.11648/j.ajls.20150303.21
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 203
EP  - 212
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20150303.21
AB  - This study evaluated the effect of Annona senegalensis on selected biochemical and testicular function indices in rats. Twenty Wistar rats (125 – 127 g) were randomly assigned into four experimental groups: A, B, C and D, (n = 5/ group). Animals in group A served as control and was administered distilled water, while animals in groups B, C, and D were given 100 mg/kg body weight (bw), 200 mg/kg bw and 300 mg/kg bw of aqueous leaf extract of Annona senegalensis (ALEAS) respectively by oral gavage for 21 days. Data revealed a significant decrease (P < 0.05) in the testis-body weight ratio and total protein content in the testes of rats administered ALEAS compared to the control. There was also a significant increase (P < 0.05) in testicular cholesterol, glycogen, malondialdhyde (MDA) and catalase (CAT) activity. Additionally, histopathological studies revealed a marked degeneration of seminiferous tubules. Overall, data from the present study suggest that aqueous leaf extract of Annona senegalensis may possess the potential to adversely affect testicular function in rat.
VL  - 3
IS  - 3
ER  -

Copy | Download