Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs

Kolawole Victor Olorunshola; Cheh Augustine Awasum; Nenadi Claire Hedima

doi:doi:10.11648/j.ajcem.20140206.14

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Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs

Kolawole Victor Olorunshola, Cheh Augustine Awasum, Nenadi Claire Hedima

Published in American Journal of Clinical and Experimental Medicine (Volume 2, Issue 6)

Received: 29 September 2014 Accepted: 7 November 2014 Published: 17 November 2014

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Abstract

This study was aimed at evaluating the effect of caffeine on biliary secretions in indigenous Nigerian dogs. A total of 6 dogs weighing 12 – 15 kg divided into 2 groups were used. The control group was fed their normal diet and water ad libitum and the treated group received 16 mg/kg of white crystalline caffeine dissolved in 10 mls of water and administered orally 8 hours prior to each surgery. Under sodium thiopentone and ketamine anaesthesia, common bile duct cannulation was done by the modified method of Rath and Hutchison. Bile was collected immediately post cannulation over a period of 48 to 72 hours. The bile samples from all dogs were analysed for bile volume, pH and electrolyte concentrations. The results showed significant increase in the bile volume in the caffeine treated group: 3.41 ± 0.85 ml compared to the control group: 1.24 ± 0.17 ml (p<0.05). The bile pH in the caffeine treated group: 7.40 ± 0.24 was significantly higher than the control group: 6.68 ± 0.18 (p< 0.05). The potassium concentration of 6.08 ± 0.49mmol/L in control group was significantly higher than the potassium concentration of 4.81 ± 0.21mmol/L in the treated group (p< 0.05). However, there was no significant change in the concentration of bicarbonate, chloride and sodium ions in the caffeine treated animals. We conclude that orally administered caffeine significantly increased bile volume and bile PH and significantly decreased bile potassium concentration in indigenous Nigerian dogs and these findings may have implication for digestion and absorption of fat soluble vitamins and a measure of liver functions.

Published in	American Journal of Clinical and Experimental Medicine (Volume 2, Issue 6)
DOI	10.11648/j.ajcem.20140206.14
Page(s)	132-136
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Caffeine, Bile Volume, Bile Secretion, Liver Function, Fat Digestion and Absorption, Indigenous Nigerian Dogs

References

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APA Style

Kolawole Victor Olorunshola, Cheh Augustine Awasum, Nenadi Claire Hedima. (2014). Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs. American Journal of Clinical and Experimental Medicine, 2(6), 132-136. https://doi.org/10.11648/j.ajcem.20140206.14

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ACS Style

Kolawole Victor Olorunshola; Cheh Augustine Awasum; Nenadi Claire Hedima. Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs. Am. J. Clin. Exp. Med. 2014, 2(6), 132-136. doi: 10.11648/j.ajcem.20140206.14

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AMA Style

Kolawole Victor Olorunshola, Cheh Augustine Awasum, Nenadi Claire Hedima. Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs. Am J Clin Exp Med. 2014;2(6):132-136. doi: 10.11648/j.ajcem.20140206.14

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@article{10.11648/j.ajcem.20140206.14,
  author = {Kolawole Victor Olorunshola and Cheh Augustine Awasum and Nenadi Claire Hedima},
  title = {Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {2},
  number = {6},
  pages = {132-136},
  doi = {10.11648/j.ajcem.20140206.14},
  url = {https://doi.org/10.11648/j.ajcem.20140206.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140206.14},
  abstract = {This study was aimed at evaluating the effect of caffeine on biliary secretions in indigenous Nigerian dogs. A total of 6 dogs weighing 12 – 15 kg divided into 2 groups were used. The control group was fed their normal diet and water ad libitum and the treated group received 16 mg/kg of white crystalline caffeine dissolved in 10 mls of water and administered orally 8 hours prior to each surgery. Under sodium thiopentone and ketamine anaesthesia, common bile duct cannulation was done by the modified method of Rath and Hutchison. Bile was collected immediately post cannulation over a period of 48 to 72 hours. The bile samples from all dogs were analysed for bile volume, pH and electrolyte concentrations. The results showed significant increase in the bile volume in the caffeine treated group: 3.41 ± 0.85 ml compared to the control group: 1.24 ± 0.17 ml (p<0.05). The bile pH in the caffeine treated group: 7.40 ± 0.24 was significantly higher than the control group: 6.68 ± 0.18 (p< 0.05). The potassium concentration of 6.08 ± 0.49mmol/L in control group was significantly higher than the potassium concentration of 4.81 ± 0.21mmol/L in the treated group (p< 0.05). However, there was no significant change in the concentration of bicarbonate, chloride and sodium ions in the caffeine treated animals. We conclude that orally administered caffeine significantly increased bile volume and bile PH and significantly decreased bile potassium concentration in indigenous Nigerian dogs and these findings may have implication for digestion and absorption of fat soluble vitamins and a measure of liver functions.},
 year = {2014}
}

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TY  - JOUR
T1  - Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs
AU  - Kolawole Victor Olorunshola
AU  - Cheh Augustine Awasum
AU  - Nenadi Claire Hedima
Y1  - 2014/11/17
PY  - 2014
N1  - https://doi.org/10.11648/j.ajcem.20140206.14
DO  - 10.11648/j.ajcem.20140206.14
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 132
EP  - 136
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20140206.14
AB  - This study was aimed at evaluating the effect of caffeine on biliary secretions in indigenous Nigerian dogs. A total of 6 dogs weighing 12 – 15 kg divided into 2 groups were used. The control group was fed their normal diet and water ad libitum and the treated group received 16 mg/kg of white crystalline caffeine dissolved in 10 mls of water and administered orally 8 hours prior to each surgery. Under sodium thiopentone and ketamine anaesthesia, common bile duct cannulation was done by the modified method of Rath and Hutchison. Bile was collected immediately post cannulation over a period of 48 to 72 hours. The bile samples from all dogs were analysed for bile volume, pH and electrolyte concentrations. The results showed significant increase in the bile volume in the caffeine treated group: 3.41 ± 0.85 ml compared to the control group: 1.24 ± 0.17 ml (p<0.05). The bile pH in the caffeine treated group: 7.40 ± 0.24 was significantly higher than the control group: 6.68 ± 0.18 (p< 0.05). The potassium concentration of 6.08 ± 0.49mmol/L in control group was significantly higher than the potassium concentration of 4.81 ± 0.21mmol/L in the treated group (p< 0.05). However, there was no significant change in the concentration of bicarbonate, chloride and sodium ions in the caffeine treated animals. We conclude that orally administered caffeine significantly increased bile volume and bile PH and significantly decreased bile potassium concentration in indigenous Nigerian dogs and these findings may have implication for digestion and absorption of fat soluble vitamins and a measure of liver functions.
VL  - 2
IS  - 6
ER  -

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Author Information

Kolawole Victor Olorunshola

Department of Human Physiology, College of Health Sciences, University of Abuja, Abuja, Nigeria
Cheh Augustine Awasum

Department of Veterinary Surgery and Medicine / Veterinary Teaching Hospital, Ahmadu Bello University, Zaria, Nigeria
Nenadi Claire Hedima

Department of Veterinary Surgery and Medicine / Veterinary Teaching Hospital, Ahmadu Bello University, Zaria, Nigeria

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APA Style

Kolawole Victor Olorunshola, Cheh Augustine Awasum, Nenadi Claire Hedima. (2014). Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs. American Journal of Clinical and Experimental Medicine, 2(6), 132-136. https://doi.org/10.11648/j.ajcem.20140206.14

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ACS Style

Kolawole Victor Olorunshola; Cheh Augustine Awasum; Nenadi Claire Hedima. Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs. Am. J. Clin. Exp. Med. 2014, 2(6), 132-136. doi: 10.11648/j.ajcem.20140206.14

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AMA Style

Kolawole Victor Olorunshola, Cheh Augustine Awasum, Nenadi Claire Hedima. Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs. Am J Clin Exp Med. 2014;2(6):132-136. doi: 10.11648/j.ajcem.20140206.14

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@article{10.11648/j.ajcem.20140206.14,
  author = {Kolawole Victor Olorunshola and Cheh Augustine Awasum and Nenadi Claire Hedima},
  title = {Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {2},
  number = {6},
  pages = {132-136},
  doi = {10.11648/j.ajcem.20140206.14},
  url = {https://doi.org/10.11648/j.ajcem.20140206.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140206.14},
  abstract = {This study was aimed at evaluating the effect of caffeine on biliary secretions in indigenous Nigerian dogs. A total of 6 dogs weighing 12 – 15 kg divided into 2 groups were used. The control group was fed their normal diet and water ad libitum and the treated group received 16 mg/kg of white crystalline caffeine dissolved in 10 mls of water and administered orally 8 hours prior to each surgery. Under sodium thiopentone and ketamine anaesthesia, common bile duct cannulation was done by the modified method of Rath and Hutchison. Bile was collected immediately post cannulation over a period of 48 to 72 hours. The bile samples from all dogs were analysed for bile volume, pH and electrolyte concentrations. The results showed significant increase in the bile volume in the caffeine treated group: 3.41 ± 0.85 ml compared to the control group: 1.24 ± 0.17 ml (p<0.05). The bile pH in the caffeine treated group: 7.40 ± 0.24 was significantly higher than the control group: 6.68 ± 0.18 (p< 0.05). The potassium concentration of 6.08 ± 0.49mmol/L in control group was significantly higher than the potassium concentration of 4.81 ± 0.21mmol/L in the treated group (p< 0.05). However, there was no significant change in the concentration of bicarbonate, chloride and sodium ions in the caffeine treated animals. We conclude that orally administered caffeine significantly increased bile volume and bile PH and significantly decreased bile potassium concentration in indigenous Nigerian dogs and these findings may have implication for digestion and absorption of fat soluble vitamins and a measure of liver functions.},
 year = {2014}
}

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TY  - JOUR
T1  - Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs
AU  - Kolawole Victor Olorunshola
AU  - Cheh Augustine Awasum
AU  - Nenadi Claire Hedima
Y1  - 2014/11/17
PY  - 2014
N1  - https://doi.org/10.11648/j.ajcem.20140206.14
DO  - 10.11648/j.ajcem.20140206.14
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 132
EP  - 136
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20140206.14
AB  - This study was aimed at evaluating the effect of caffeine on biliary secretions in indigenous Nigerian dogs. A total of 6 dogs weighing 12 – 15 kg divided into 2 groups were used. The control group was fed their normal diet and water ad libitum and the treated group received 16 mg/kg of white crystalline caffeine dissolved in 10 mls of water and administered orally 8 hours prior to each surgery. Under sodium thiopentone and ketamine anaesthesia, common bile duct cannulation was done by the modified method of Rath and Hutchison. Bile was collected immediately post cannulation over a period of 48 to 72 hours. The bile samples from all dogs were analysed for bile volume, pH and electrolyte concentrations. The results showed significant increase in the bile volume in the caffeine treated group: 3.41 ± 0.85 ml compared to the control group: 1.24 ± 0.17 ml (p<0.05). The bile pH in the caffeine treated group: 7.40 ± 0.24 was significantly higher than the control group: 6.68 ± 0.18 (p< 0.05). The potassium concentration of 6.08 ± 0.49mmol/L in control group was significantly higher than the potassium concentration of 4.81 ± 0.21mmol/L in the treated group (p< 0.05). However, there was no significant change in the concentration of bicarbonate, chloride and sodium ions in the caffeine treated animals. We conclude that orally administered caffeine significantly increased bile volume and bile PH and significantly decreased bile potassium concentration in indigenous Nigerian dogs and these findings may have implication for digestion and absorption of fat soluble vitamins and a measure of liver functions.
VL  - 2
IS  - 6
ER  -

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