Objectives: Investigation with the crude ethanolic extract of Argyria captiformis leaves was carried out to evaluate its possible thrombolysis and to analyze in –vivo neuro-pharmacological effects as anxiety is a particular form of behavioral inhibition that occurs in response to novel environment events and also phytochemical screening of plant extract. Method: Ethanolic extract of Argyria captiformis Leaves was assessed for sedative and anxiolytic activity on Swiss albino mice and Thrombolytic activity was assessed with human blood and also phytochemical screening test was done by various chemical reagents. Sedative activity was evaluated by using hole cross, open field, thiopental sodium-induced sleeping time and anxiolytic property was evaluated by elevated-plus maze(EPM) tests at 400mg/kg while the peripheral and thrombolytic activity determined by percentage of clot lysis. Result: In anxiolytic study, the extract displayed increased percentage of entry into open arm at the dose of 400mg/kg. The extract produced a significant (P<0.01) increase in sleeping duration and reduction of onset of sleep compared to sodium thiopental at doses (400 mg/kg) .The extract (400 mg/kg) also showed suppression of motor activity and exploratory activity of the mice in both open field and hole cross test. Argyria captiformis alone & Argyria captiformis in combination with Streptokinase demonstrated 38.19±4.76% & 77.45±2.97% clot lysis effect respectively & revealed significant with comparison to both the control agent. The presence of tannins, glycosides, saponins, flavonoids, cardiac glycosides, and phytosterols was determined. Conclusion: The pharmacological profiles of the present investigation of the ethanol extract of A. captiformis indicate that the extract possess good CNS depressant and exhibited considerable thrombolytic as it significantly reduced locomotion, onset of sleep, increased duration of sleep and also presence of, glycosides, cardiac glycosides, saponins, flavonoids ,tannins and phytosterols.
| Published in | American Journal of Biomedical and Life Sciences (Volume 3, Issue 2) |
| DOI | 10.11648/j.ajbls.20150302.12 |
| Page(s) | 16-24 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Sedative and Anxiolytic Activity, Phytochemical, Argyria Captiformis, Thrombolytic Activity, % Lysis of Clot
| [1] | Buller R, Legrand V. Novel treatments for anxiety and depression: hurdles in bringing them to the market. Drug Discovery Today 2001; 6: 1220-1230. |
| [2] | Yadav AV, Kawale LA, Nade VS. Effect of Morus Alba L. (mulberry) leaves on anxiety in mice. Indian J Pharmacol 2008; 40: 32-36. |
| [3] | Titov N, Andrews G, Kemp A, Robinson E. Characteristics of adults with anxiety or depression treated at an internet clinic: comparison with a national survey and an outpatient clinic. PLOS ONE 2010; 5(5): e10885. |
| [4] | Woode E, Abotsi WK, Mensah AY. Anxiolytic-and antidepressant-like effects of an ethanolic extract of the aerial parts of Hilleria latifolia (Lam.) H. Walt. in mice. J Nat Pharm 2011; 2: 62-71. |
| [5] | Rabbani M, Sajjadi SE, Mohammadi A. Evaluation of the anxiolytic effect of Nepeta persica Boiss. In mice. Evid-Based Complement Alternat Med 2008; 5(2): 181-186. |
| [6] | Raihan MO, Habib MR, Brishti A, Rahman MM. Sedative and anxiolytic effects of the methanolic extract of Leea indica Burm. |
| [7] | Rahman H, Elumalai A, Eswaraiah MC, Bardalai D. Evaluation of anxiolytic activity of ethanolic extract of Pisonia grandis R. Br |
| [8] | Newman M, Lhuillier A, Poulsen AD. Checklist of the Zingiberaceae of Malesia. Blumea Suppl 2004; 16: 166. |
| [9] | Wu DL. Flora of China.Vol.16, Fascicle 2. Beijing: Science Press; 1981, p. 67-106. |
| [10] | Thrombus.2011.Thrombus.[ONLINEAvailableat: http://adam.about.net/encyclopedia/Thrombus.htm. [Accessed 21 August 2011]. |
| [11] | D.R. P.N. Bennett; Clinical Pharmacology: Seventh Edition 1992: Page 483 |
| [12] | Nicolini FA, Nichols WW, Mehta JL, Saldeen TG, Schofield R, Ross M, Player DW, Pohl GB, Mattsson C. Sustained reflow in dogs with coronary thrombosis with K2P, a novel mutant of tissue plasminogen activator. J Am Coll Cardiol. 1992; 20:228–235. |
| [13] | Adams DS, Griffin LA, Nachajko WR, Reddy VB, Wei CM. A synthetic DNA Encoding a modified human urokinase resistant to inhibition by serum plasminogen activator inhibitor. J Biol Chem. 1991; 266:8476–8482. |
| [14] | Lijnen HR, Vanhoef B, DeCock F, Okada K, Ueshima S, Matsuo O. On the mechanism of fibrin-specific plasminogen activation by staphylokinase. J Biol Chem. 1991; 266:11826–11832. |
| [15] | Marder VJ. Recombinant streptokinase – opportunity for an improved agent. Blood Coagul Fibrinolysis. 1993; 4:1039–1040. |
| [16] | Wu DH, Shi GY, Chuang WJ, Hsu JM, Young KC, Chang CW. Coiled coil region of streptokinase gamma-domain is essential for plasminogen activation. J Biol Chem. 2001; 276:15025–15033. doi: 10.1074/jbc.M005935200. |
| [17] | Harborne JB (1998). Phytochemical Methods. Chapman and Hall, London. |
| [18] | Giuseppina Basta, Cristiana Lupi, Guido Lazzerini, Piero Chiarelli, Antonio L’Abbate, Daniele Rovai: Therapeutic effect of diagnostic ultrasound on enzymatic thrombolysis. An in vitro study on blood of normal subjects and patients with coronary artery disease. Thromb Haemost 2004, 91:1078-1083. |
| [19] | www.Ethnobotanical Database of Bangladesh (EDB) 3.1 Plants of Bangladesh |
| [20] | Rahman, M.A., Uddin, S.B., Wilcock, C.C., 2007. Medicinal Plants used by Chakma tribe in Hill Tracts districts of Bangladesh. Indian Journal of Traditional Knowledge. 6(3):508-517. |
| [21] | Gupta BD, Dandiya PC, Gupta ML. A psychopharmacological analysis of behavior in rat. Jpn J Pharmacol 1971; 21: 293. |
| [22] | Lister RG. The use of a plus-maze to measure anxiety in the mouse. Psychopharmacology (Berl) 1987; 92: 180-185. |
| [23] | Pellow S, Chopin P, File SE, Briley M. Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 1985; 14: 149-167. |
| [24] | Ferrini R, Miragoli G, Taccardi B. Neuro-pharmacological studies on SB 5833, a new psychotherapeutic agent of the benzodiazepine class. Arzneim-Forsch (Drug Res) 1974; 24: 2029-2032. |
| [25] | Takagi K, Watanabe M, Saito H. Studies on the spontaneous movement of animals by the hole cross test; effect of 2-dimethylaminoethanol and its acyl esters on the central nervous system. Jpn J Pharmacol 1971; 21: 797-810. |
| [26] | Sweta Prasad, Rajpal Singh Kashyap, Jayant Y Deopujari, Hemant J Purohit, Girdhar M Taori and Hatim F Daginawala. Effect of Fagonia Arabica (Dhamasa) on in vitro thrombolysis, BMC Complementary and Alternative Medicine 2007, 7:36 doi: 10.1186/1472-6882-7-36 |
| [27] | Prashant Tiwari, Bimlesh Kumar,Mandeep Kaur,Gurpreet Kaur,Harleen Kaur Phytochemical screening and Extraction,2011.International Pharmaceutica Sciencia journal, vol.1,Issue 1 (2011) |
| [28] | http://www.ukessays.co.uk/essays/biology/phytochemical-analysis.php |
| [29] | Dhonnchadha BAN, Bourin M, Hascoet M. Anxiolytic-like effects of 5-HT2 ligands on three mouse models of anxiety. Behav Brain Res 2011; 140: 203-214. |
| [30] | Subramanian N, Jothimanivannan C, Kumar RS, Kameshwaran S. Evaluation of anti-anxiety activity of Justicia gendarussa burm. Pharmacologia 2013; 4(5): 404-407. |
| [31] | Thakur VD, Mengi SA. Neuropharmacological profile of Eclipta alba (Linn.) Hassk. J Ethnopharmacol 2005; 102(1): 23-31. |
| [32] | Raquibul Hasan SM, Hossain MM, Akter R, Jamila M, Mazumder EHM, Rahman S. Sedative and anxiolytic effects of different fractions of the Commelina benghalensis Linn. Drug Discov Ther 2009; 3: 221-227. |
| [33] | Sen AK, Bose S, Dutta SK. Comparative evaluation of CNS depressant activity of the flavonoid fractions from the fresh leaves and flowers of Ixora coccinea Linn. J Pharma Sci Tech 2011; 1(1): 54-56. |
| [34] | Hossain MM, Biva IJ, Jahangir R, Vhuiyan MMI. Central nervous system depressant and analgesic activity of Aphanamixis polystachya (Wall.) parker leaf extract in mice. Afr J Pharm Pharmacol 2009; 3(5): 282-286. |
| [35] | Fernández SP, Wasowski C, Loscalzo LM, Granger RE, Johnston GA, Paladini AC, et al. Central nervous system depressant action of flavonoid glycosides. Eur J Pharmacol 2006; 539: 168-176. |
| [36] | Jäger AK, Saaby L. Flavonoids and the CNS. Molecules 2011; 16: 1471-1485. |
| [37] | Johnston GA. GABA (A) receptor channel pharmacology. Curr Pharm Des 2005; 11: 1867-1885. |
| [38] | Hanrahan JR, Chebib M, Johnston GAR. Flavonoid modulation of GABAA receptors. Br J Pharmacol 2011; 163(2): 234-245. |
| [39] | Thirupathy KP, Tulshkar A, Vijaya C. Neuropharmacological activity of Lippia nodiflora Linn. Pharmacognosy Res 2011; 3(3): 194-200. |
| [40] | Boudjeltia KZ, Cauchie P, Remacle C, Guillaume M, Brohee D, Hubert JL, Vanhaeverbeek M: A new device for measurement of fibrin clot lysis: application to the Euglobulin Clot Lysis Time. BMC Biotechnology 2002, 2:8. |
| [41] | Greenberg RK, Ouriel K, Srivastava S, Shortell C, Ivancev K, Waldman D, Illig K, Green R: Mechanical versus Chemical Thrombolysis: An In Vitro Differentiation of Thrombolytic Mechanisms. Journal of Vascular and Interventional Radiology 2000, 11:199-205. |
| [42] | Erzsebet Komorowicz, Krasimir Kolev, Istvan Lerant, Raymund Machovich: Flow Rate- Modulated Dissolution of Fibrin with Clot-Embedded and Circulating Proteases. Circulation Research 1998, 82:1102-1108. |
APA Style
Saiful Islam, Naymul Karim, Imam Hasan, Md Hossan Sakib, Md. Harun-Or- Rashid, et al. (2015). Investigation of In-Vivo Neuropharmacological and In-Vitro Thrombolytic Activity & Phytochemical Analysis of Ethanolic Extract of Argyria Captiformis Leaves. American Journal of Biomedical and Life Sciences, 3(2), 16-24. https://doi.org/10.11648/j.ajbls.20150302.12
ACS Style
Saiful Islam; Naymul Karim; Imam Hasan; Md Hossan Sakib; Md. Harun-Or- Rashid, et al. Investigation of In-Vivo Neuropharmacological and In-Vitro Thrombolytic Activity & Phytochemical Analysis of Ethanolic Extract of Argyria Captiformis Leaves. Am. J. Biomed. Life Sci. 2015, 3(2), 16-24. doi: 10.11648/j.ajbls.20150302.12
AMA Style
Saiful Islam, Naymul Karim, Imam Hasan, Md Hossan Sakib, Md. Harun-Or- Rashid, et al. Investigation of In-Vivo Neuropharmacological and In-Vitro Thrombolytic Activity & Phytochemical Analysis of Ethanolic Extract of Argyria Captiformis Leaves. Am J Biomed Life Sci. 2015;3(2):16-24. doi: 10.11648/j.ajbls.20150302.12
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ajbls.20150302.12,
author = {Saiful Islam and Naymul Karim and Imam Hasan and Md Hossan Sakib and Md. Harun-Or- Rashid and Sadequr Rahman and Masudur Rahman},
title = {Investigation of In-Vivo Neuropharmacological and In-Vitro Thrombolytic Activity & Phytochemical Analysis of Ethanolic Extract of Argyria Captiformis Leaves},
journal = {American Journal of Biomedical and Life Sciences},
volume = {3},
number = {2},
pages = {16-24},
doi = {10.11648/j.ajbls.20150302.12},
url = {https://doi.org/10.11648/j.ajbls.20150302.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150302.12},
abstract = {Objectives: Investigation with the crude ethanolic extract of Argyria captiformis leaves was carried out to evaluate its possible thrombolysis and to analyze in –vivo neuro-pharmacological effects as anxiety is a particular form of behavioral inhibition that occurs in response to novel environment events and also phytochemical screening of plant extract. Method: Ethanolic extract of Argyria captiformis Leaves was assessed for sedative and anxiolytic activity on Swiss albino mice and Thrombolytic activity was assessed with human blood and also phytochemical screening test was done by various chemical reagents. Sedative activity was evaluated by using hole cross, open field, thiopental sodium-induced sleeping time and anxiolytic property was evaluated by elevated-plus maze(EPM) tests at 400mg/kg while the peripheral and thrombolytic activity determined by percentage of clot lysis. Result: In anxiolytic study, the extract displayed increased percentage of entry into open arm at the dose of 400mg/kg. The extract produced a significant (P<0.01) increase in sleeping duration and reduction of onset of sleep compared to sodium thiopental at doses (400 mg/kg) .The extract (400 mg/kg) also showed suppression of motor activity and exploratory activity of the mice in both open field and hole cross test. Argyria captiformis alone & Argyria captiformis in combination with Streptokinase demonstrated 38.19±4.76% & 77.45±2.97% clot lysis effect respectively & revealed significant with comparison to both the control agent. The presence of tannins, glycosides, saponins, flavonoids, cardiac glycosides, and phytosterols was determined. Conclusion: The pharmacological profiles of the present investigation of the ethanol extract of A. captiformis indicate that the extract possess good CNS depressant and exhibited considerable thrombolytic as it significantly reduced locomotion, onset of sleep, increased duration of sleep and also presence of, glycosides, cardiac glycosides, saponins, flavonoids ,tannins and phytosterols.},
year = {2015}
}
TY - JOUR T1 - Investigation of In-Vivo Neuropharmacological and In-Vitro Thrombolytic Activity & Phytochemical Analysis of Ethanolic Extract of Argyria Captiformis Leaves AU - Saiful Islam AU - Naymul Karim AU - Imam Hasan AU - Md Hossan Sakib AU - Md. Harun-Or- Rashid AU - Sadequr Rahman AU - Masudur Rahman Y1 - 2015/04/10 PY - 2015 N1 - https://doi.org/10.11648/j.ajbls.20150302.12 DO - 10.11648/j.ajbls.20150302.12 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 16 EP - 24 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20150302.12 AB - Objectives: Investigation with the crude ethanolic extract of Argyria captiformis leaves was carried out to evaluate its possible thrombolysis and to analyze in –vivo neuro-pharmacological effects as anxiety is a particular form of behavioral inhibition that occurs in response to novel environment events and also phytochemical screening of plant extract. Method: Ethanolic extract of Argyria captiformis Leaves was assessed for sedative and anxiolytic activity on Swiss albino mice and Thrombolytic activity was assessed with human blood and also phytochemical screening test was done by various chemical reagents. Sedative activity was evaluated by using hole cross, open field, thiopental sodium-induced sleeping time and anxiolytic property was evaluated by elevated-plus maze(EPM) tests at 400mg/kg while the peripheral and thrombolytic activity determined by percentage of clot lysis. Result: In anxiolytic study, the extract displayed increased percentage of entry into open arm at the dose of 400mg/kg. The extract produced a significant (P<0.01) increase in sleeping duration and reduction of onset of sleep compared to sodium thiopental at doses (400 mg/kg) .The extract (400 mg/kg) also showed suppression of motor activity and exploratory activity of the mice in both open field and hole cross test. Argyria captiformis alone & Argyria captiformis in combination with Streptokinase demonstrated 38.19±4.76% & 77.45±2.97% clot lysis effect respectively & revealed significant with comparison to both the control agent. The presence of tannins, glycosides, saponins, flavonoids, cardiac glycosides, and phytosterols was determined. Conclusion: The pharmacological profiles of the present investigation of the ethanol extract of A. captiformis indicate that the extract possess good CNS depressant and exhibited considerable thrombolytic as it significantly reduced locomotion, onset of sleep, increased duration of sleep and also presence of, glycosides, cardiac glycosides, saponins, flavonoids ,tannins and phytosterols. VL - 3 IS - 2 ER -
Email: