Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women

Rebelo Ana Cristina Silva; Melo Aryanne Batista Soares; Salviati Mariana Rodrigues; Verlengia Rozangela; Vale Arthur Ferreira Do; Nogueira Yanley Lucio; Tamburús Nayara Yamada; Kunz Vandeni Clarice; Silva Ester Da

doi:doi:10.11648/j.ajbio.20150305.15

Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women

Rebelo Ana Cristina Silva, Melo Aryanne Batista Soares, Salviati Mariana Rodrigues, Verlengia Rozangela, Vale Arthur Ferreira Do, Nogueira Yanley Lucio, Tamburús Nayara Yamada, Kunz Vandeni Clarice, Silva Ester Da

Published in American Journal of BioScience (Volume 3, Issue 5)

Received: 12 August 2015 Accepted: 17 August 2015 Published: 29 August 2015

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Abstract

Background: Studies have shown an interaction between the autonomic heart rate (HR) modulation and genetic polymorphisms. Nevertheless, it is yet unclear how a particular genetic polymorphism may determine a pattern of autonomic HR control, especially regarding the role of physical exercise. The objective was to investigate the influence of of Beta-2-Adrenergic (β2-AR) Gln27Glu polymorphisms and exercise on HRV of young Brazilian women. Methods: The group was selected as Sedentary Group (SG; n = 88) and physically Active Group (AG; n = 99). The β2-AR polymorphisms were analyzed using PCR-RFLP. The amplicons were subjected to electrophoresis on a 10% acrylamide gel and stained with ethidium bromide. The HR was registered in real time for 15 minutes, in the supine-position. The heart rate variability (HRV) was analysed using Shannon’s entropy (SE), conditional entropy (complexity index [CInd] and normalized CInd [NCI]), and symbolic analysis (0V%, 1V%, 2LV%, and 2ULV%). Statistical analysis: Analysis of variance (ANOVA) with Fisher's Least. Results: AG presented higher values for SE and 2ULV%, and lower values for 0V% when compared to SG (p<0.05). There was no signiﬁcant difference of any index among genotypes both from the complexity and symbolic analysis. The interaction between genotype and exercise did not significantly affect HRV. Conclusion: These results do not support the concept that the β2-AR Gln27Glu polymorphisms affects the HRV indexes in physically active or sedentary women.

Published in	American Journal of BioScience (Volume 3, Issue 5)
DOI	10.11648/j.ajbio.20150305.15
Page(s)	190-196
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

β₂-Adrenergic Polymorphism, Heart Rate Variability, Non-Linear Analysis, Women, Exercise

References

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Rebelo Ana Cristina Silva, Melo Aryanne Batista Soares, Salviati Mariana Rodrigues, Verlengia Rozangela, Vale Arthur Ferreira Do, et al. (2015). Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women. American Journal of BioScience, 3(5), 190-196. https://doi.org/10.11648/j.ajbio.20150305.15

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ACS Style

Rebelo Ana Cristina Silva; Melo Aryanne Batista Soares; Salviati Mariana Rodrigues; Verlengia Rozangela; Vale Arthur Ferreira Do, et al. Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women. Am. J. BioScience 2015, 3(5), 190-196. doi: 10.11648/j.ajbio.20150305.15

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AMA Style

Rebelo Ana Cristina Silva, Melo Aryanne Batista Soares, Salviati Mariana Rodrigues, Verlengia Rozangela, Vale Arthur Ferreira Do, et al. Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women. Am J BioScience. 2015;3(5):190-196. doi: 10.11648/j.ajbio.20150305.15

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@article{10.11648/j.ajbio.20150305.15,
  author = {Rebelo Ana Cristina Silva and Melo Aryanne Batista Soares and Salviati Mariana Rodrigues and Verlengia Rozangela and Vale Arthur Ferreira Do and Nogueira Yanley Lucio and Tamburús Nayara Yamada and Kunz Vandeni Clarice and Silva Ester Da},
  title = {Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women},
  journal = {American Journal of BioScience},
  volume = {3},
  number = {5},
  pages = {190-196},
  doi = {10.11648/j.ajbio.20150305.15},
  url = {https://doi.org/10.11648/j.ajbio.20150305.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20150305.15},
  abstract = {Background: Studies have shown an interaction between the autonomic heart rate (HR) modulation and genetic polymorphisms. Nevertheless, it is yet unclear how a particular genetic polymorphism may determine a pattern of autonomic HR control, especially regarding the role of physical exercise. The objective was to investigate the influence of of Beta-2-Adrenergic (β2-AR) Gln27Glu polymorphisms and exercise on HRV of young Brazilian women. Methods: The group was selected as Sedentary Group (SG; n = 88) and physically Active Group (AG; n = 99). The β2-AR polymorphisms were analyzed using PCR-RFLP. The amplicons were subjected to electrophoresis on a 10% acrylamide gel and stained with ethidium bromide. The HR was registered in real time for 15 minutes, in the supine-position. The heart rate variability (HRV) was analysed using Shannon’s entropy (SE), conditional entropy (complexity index [CInd] and normalized CInd [NCI]), and symbolic analysis (0V%, 1V%, 2LV%, and 2ULV%). Statistical analysis: Analysis of variance (ANOVA) with Fisher's Least. Results: AG presented higher values for SE and 2ULV%, and lower values for 0V% when compared to SG (p<0.05). There was no signiﬁcant difference of any index among genotypes both from the complexity and symbolic analysis. The interaction between genotype and exercise did not significantly affect HRV. Conclusion: These results do not support the concept that the β2-AR Gln27Glu polymorphisms affects the HRV indexes in physically active or sedentary women.},
 year = {2015}
}

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TY - JOUR
T1 - Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women
AU - Rebelo Ana Cristina Silva
AU - Melo Aryanne Batista Soares
AU - Salviati Mariana Rodrigues
AU - Verlengia Rozangela
AU - Vale Arthur Ferreira Do
AU - Nogueira Yanley Lucio
AU - Tamburús Nayara Yamada
AU - Kunz Vandeni Clarice
AU - Silva Ester Da
Y1 - 2015/08/29
PY - 2015
N1 - https://doi.org/10.11648/j.ajbio.20150305.15
DO - 10.11648/j.ajbio.20150305.15
T2 - American Journal of BioScience
JF - American Journal of BioScience
JO - American Journal of BioScience
SP - 190
EP - 196
PB - Science Publishing Group
SN - 2330-0167
UR - https://doi.org/10.11648/j.ajbio.20150305.15
AB - Background: Studies have shown an interaction between the autonomic heart rate (HR) modulation and genetic polymorphisms. Nevertheless, it is yet unclear how a particular genetic polymorphism may determine a pattern of autonomic HR control, especially regarding the role of physical exercise. The objective was to investigate the influence of of Beta-2-Adrenergic (β2-AR) Gln27Glu polymorphisms and exercise on HRV of young Brazilian women. Methods: The group was selected as Sedentary Group (SG; n = 88) and physically Active Group (AG; n = 99). The β2-AR polymorphisms were analyzed using PCR-RFLP. The amplicons were subjected to electrophoresis on a 10% acrylamide gel and stained with ethidium bromide. The HR was registered in real time for 15 minutes, in the supine-position. The heart rate variability (HRV) was analysed using Shannon’s entropy (SE), conditional entropy (complexity index [CInd] and normalized CInd [NCI]), and symbolic analysis (0V%, 1V%, 2LV%, and 2ULV%). Statistical analysis: Analysis of variance (ANOVA) with Fisher's Least. Results: AG presented higher values for SE and 2ULV%, and lower values for 0V% when compared to SG (p<0.05). There was no signiﬁcant difference of any index among genotypes both from the complexity and symbolic analysis. The interaction between genotype and exercise did not significantly affect HRV. Conclusion: These results do not support the concept that the β2-AR Gln27Glu polymorphisms affects the HRV indexes in physically active or sedentary women.
VL - 3
IS - 5
ER -

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Author Information

Rebelo Ana Cristina Silva

Department of Morphology, Biological Sciences Institute, Federal University of Goiás, Goiania, Brazil
Melo Aryanne Batista Soares

Center for Neuroscience and Cardiovascular Physiology Research, Department of Physiological Sciences, Biological Sciences Institute, Federal University of Goiás, Goiania, Brazil
Salviati Mariana Rodrigues

Humana Performance Research Group - College of Health Sciences (FACIS), Methodist University of Piracicaba (UNIMEP), Piracicaba, Brazil
Verlengia Rozangela

Humana Performance Research Group - College of Health Sciences (FACIS), Methodist University of Piracicaba (UNIMEP), Piracicaba, Brazil
Vale Arthur Ferreira Do

Department of Morphology, Biological Sciences Institute, Federal University of Goiás, Goiania, Brazil
Nogueira Yanley Lucio

Faculty of Medicine, Federal University of Goiás, Goiania, Brazil
Tamburús Nayara Yamada

Department of Physiotherapy, Federal University of Sao Carlos, Sao Carlos, Brazil
Kunz Vandeni Clarice

Adventist University of Sao Paulo, Engenheiro Coelho, Sao Paulo, Brazil
Silva Ester Da

Department of Physiotherapy, Federal University of Sao Carlos, Sao Carlos, Brazil

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APA Style

Rebelo Ana Cristina Silva, Melo Aryanne Batista Soares, Salviati Mariana Rodrigues, Verlengia Rozangela, Vale Arthur Ferreira Do, et al. (2015). Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women. American Journal of BioScience, 3(5), 190-196. https://doi.org/10.11648/j.ajbio.20150305.15

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Rebelo Ana Cristina Silva; Melo Aryanne Batista Soares; Salviati Mariana Rodrigues; Verlengia Rozangela; Vale Arthur Ferreira Do, et al. Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women. Am. J. BioScience 2015, 3(5), 190-196. doi: 10.11648/j.ajbio.20150305.15

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AMA Style

Rebelo Ana Cristina Silva, Melo Aryanne Batista Soares, Salviati Mariana Rodrigues, Verlengia Rozangela, Vale Arthur Ferreira Do, et al. Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women. Am J BioScience. 2015;3(5):190-196. doi: 10.11648/j.ajbio.20150305.15

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@article{10.11648/j.ajbio.20150305.15,
  author = {Rebelo Ana Cristina Silva and Melo Aryanne Batista Soares and Salviati Mariana Rodrigues and Verlengia Rozangela and Vale Arthur Ferreira Do and Nogueira Yanley Lucio and Tamburús Nayara Yamada and Kunz Vandeni Clarice and Silva Ester Da},
  title = {Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women},
  journal = {American Journal of BioScience},
  volume = {3},
  number = {5},
  pages = {190-196},
  doi = {10.11648/j.ajbio.20150305.15},
  url = {https://doi.org/10.11648/j.ajbio.20150305.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20150305.15},
  abstract = {Background: Studies have shown an interaction between the autonomic heart rate (HR) modulation and genetic polymorphisms. Nevertheless, it is yet unclear how a particular genetic polymorphism may determine a pattern of autonomic HR control, especially regarding the role of physical exercise. The objective was to investigate the influence of of Beta-2-Adrenergic (β2-AR) Gln27Glu polymorphisms and exercise on HRV of young Brazilian women. Methods: The group was selected as Sedentary Group (SG; n = 88) and physically Active Group (AG; n = 99). The β2-AR polymorphisms were analyzed using PCR-RFLP. The amplicons were subjected to electrophoresis on a 10% acrylamide gel and stained with ethidium bromide. The HR was registered in real time for 15 minutes, in the supine-position. The heart rate variability (HRV) was analysed using Shannon’s entropy (SE), conditional entropy (complexity index [CInd] and normalized CInd [NCI]), and symbolic analysis (0V%, 1V%, 2LV%, and 2ULV%). Statistical analysis: Analysis of variance (ANOVA) with Fisher's Least. Results: AG presented higher values for SE and 2ULV%, and lower values for 0V% when compared to SG (p<0.05). There was no signiﬁcant difference of any index among genotypes both from the complexity and symbolic analysis. The interaction between genotype and exercise did not significantly affect HRV. Conclusion: These results do not support the concept that the β2-AR Gln27Glu polymorphisms affects the HRV indexes in physically active or sedentary women.},
 year = {2015}
}

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