‘Yeheb’ (Cordeauxia edulis Hemsl) is a multipurpose and evergreen shrub and endemic to southeastern corner of Ethiopia and Somalia. It is adapted to low and irregular rainfall and survives a very long dry season. It has enormous economic and food security role to the pastoralist of Somali in Ethiopia. However, the plant is threatened with extinction due to overexploitation and its’ poor natural regeneration capacity. In addition, ‘yeheb’ is usually reported having limited reproductive capacities and often have very specific and limited conditions for seed germination, flowering and seed shelf life. Therefore, to overcome these propagation challenges, an experiment was conducted with the aim of developing a protocol for the in vitro regeneration of ‘yeheb’ from cotyledonary node. The result of these studies revealed that seed was washed by 5% sodium hypochlorite for ten min in aseptic condition found to be more effective in surface sterilization. The sterilized seed cultured on half strength of Gamborg (B5) medium was found to be the most suitable medium for germination (26.67%).The highest shoot initiation percentage (89 % of explants produces shoots), number of shoots per explant and number of leaf per shoot were obtained from cotyledonary node explants cultured on Murashige and Skoog (MS) media supplemented with 2.00 mg. l-1 N6-benzylaminopurine (BAP) within nine weeks. While, the highest shoot length and shoot fresh weight were recorded from control (free BAP) and 6.00 mg. l-1 BAP, respectively. The highest shoot multiplication (4.56 number of shoot induced) and elongation (2.97cm) were obtained from the induced shoot were cut and placed on MS media supplemented with 2.00 mg. l 1BAP+6.00 mg. l-1of gibberellic acid (GA3) and free BAP+6.00 mg. l-1 of GA3, respectively. The elongated shoots were transferred to different media supplemented with various types and levels of hormones but none of them induced root. As a conclusion, this is the first attempt for direct in vitro regeneration of C. edulis and permissible result for cryopreservation.
| Published in | Agriculture, Forestry and Fisheries (Volume 3, Issue 6) |
| DOI | 10.11648/j.aff.20140306.13 |
| Page(s) | 452-458 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
BAP, Cotyledonary Node, Germination, In Vitro, GA3 Shoot Induction, Shoot Elongation
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APA Style
Yohannes Seyoum, Firew Mekbib. (2014). In Vitro Germination and Direct Shoot Induction of Yeheb (Cordeauxia Edulis Hemsl.). Agriculture, Forestry and Fisheries, 3(6), 452-458. https://doi.org/10.11648/j.aff.20140306.13
ACS Style
Yohannes Seyoum; Firew Mekbib. In Vitro Germination and Direct Shoot Induction of Yeheb (Cordeauxia Edulis Hemsl.). Agric. For. Fish. 2014, 3(6), 452-458. doi: 10.11648/j.aff.20140306.13
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Download@article{10.11648/j.aff.20140306.13,
author = {Yohannes Seyoum and Firew Mekbib},
title = {In Vitro Germination and Direct Shoot Induction of Yeheb (Cordeauxia Edulis Hemsl.)},
journal = {Agriculture, Forestry and Fisheries},
volume = {3},
number = {6},
pages = {452-458},
doi = {10.11648/j.aff.20140306.13},
url = {https://doi.org/10.11648/j.aff.20140306.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20140306.13},
abstract = {‘Yeheb’ (Cordeauxia edulis Hemsl) is a multipurpose and evergreen shrub and endemic to southeastern corner of Ethiopia and Somalia. It is adapted to low and irregular rainfall and survives a very long dry season. It has enormous economic and food security role to the pastoralist of Somali in Ethiopia. However, the plant is threatened with extinction due to overexploitation and its’ poor natural regeneration capacity. In addition, ‘yeheb’ is usually reported having limited reproductive capacities and often have very specific and limited conditions for seed germination, flowering and seed shelf life. Therefore, to overcome these propagation challenges, an experiment was conducted with the aim of developing a protocol for the in vitro regeneration of ‘yeheb’ from cotyledonary node. The result of these studies revealed that seed was washed by 5% sodium hypochlorite for ten min in aseptic condition found to be more effective in surface sterilization. The sterilized seed cultured on half strength of Gamborg (B5) medium was found to be the most suitable medium for germination (26.67%).The highest shoot initiation percentage (89 % of explants produces shoots), number of shoots per explant and number of leaf per shoot were obtained from cotyledonary node explants cultured on Murashige and Skoog (MS) media supplemented with 2.00 mg. l-1 N6-benzylaminopurine (BAP) within nine weeks. While, the highest shoot length and shoot fresh weight were recorded from control (free BAP) and 6.00 mg. l-1 BAP, respectively. The highest shoot multiplication (4.56 number of shoot induced) and elongation (2.97cm) were obtained from the induced shoot were cut and placed on MS media supplemented with 2.00 mg. l 1BAP+6.00 mg. l-1of gibberellic acid (GA3) and free BAP+6.00 mg. l-1 of GA3, respectively. The elongated shoots were transferred to different media supplemented with various types and levels of hormones but none of them induced root. As a conclusion, this is the first attempt for direct in vitro regeneration of C. edulis and permissible result for cryopreservation.},
year = {2014}
}
TY - JOUR T1 - In Vitro Germination and Direct Shoot Induction of Yeheb (Cordeauxia Edulis Hemsl.) AU - Yohannes Seyoum AU - Firew Mekbib Y1 - 2014/12/18 PY - 2014 N1 - https://doi.org/10.11648/j.aff.20140306.13 DO - 10.11648/j.aff.20140306.13 T2 - Agriculture, Forestry and Fisheries JF - Agriculture, Forestry and Fisheries JO - Agriculture, Forestry and Fisheries SP - 452 EP - 458 PB - Science Publishing Group SN - 2328-5648 UR - https://doi.org/10.11648/j.aff.20140306.13 AB - ‘Yeheb’ (Cordeauxia edulis Hemsl) is a multipurpose and evergreen shrub and endemic to southeastern corner of Ethiopia and Somalia. It is adapted to low and irregular rainfall and survives a very long dry season. It has enormous economic and food security role to the pastoralist of Somali in Ethiopia. However, the plant is threatened with extinction due to overexploitation and its’ poor natural regeneration capacity. In addition, ‘yeheb’ is usually reported having limited reproductive capacities and often have very specific and limited conditions for seed germination, flowering and seed shelf life. Therefore, to overcome these propagation challenges, an experiment was conducted with the aim of developing a protocol for the in vitro regeneration of ‘yeheb’ from cotyledonary node. The result of these studies revealed that seed was washed by 5% sodium hypochlorite for ten min in aseptic condition found to be more effective in surface sterilization. The sterilized seed cultured on half strength of Gamborg (B5) medium was found to be the most suitable medium for germination (26.67%).The highest shoot initiation percentage (89 % of explants produces shoots), number of shoots per explant and number of leaf per shoot were obtained from cotyledonary node explants cultured on Murashige and Skoog (MS) media supplemented with 2.00 mg. l-1 N6-benzylaminopurine (BAP) within nine weeks. While, the highest shoot length and shoot fresh weight were recorded from control (free BAP) and 6.00 mg. l-1 BAP, respectively. The highest shoot multiplication (4.56 number of shoot induced) and elongation (2.97cm) were obtained from the induced shoot were cut and placed on MS media supplemented with 2.00 mg. l 1BAP+6.00 mg. l-1of gibberellic acid (GA3) and free BAP+6.00 mg. l-1 of GA3, respectively. The elongated shoots were transferred to different media supplemented with various types and levels of hormones but none of them induced root. As a conclusion, this is the first attempt for direct in vitro regeneration of C. edulis and permissible result for cryopreservation. VL - 3 IS - 6 ER -
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