Volume 4, Issue 2, December 2020, Page: 32-35
Determination of Tetracycline Residues in Honey from Tabora and Singida Regions Tanzania Produced Using Modern Beehives
Mohamed Hamed Sango Ally, Department of Physiology, Biochemistry, Pharmacology and Toxicology, Sokoine University of Agriculture, Morogoro, Tanzania
Mnyakavilli Anna, Department of Chemistry and Physics, Sokoine University of Agriculture, Morogoro, Tanzania
Received: Sep. 18, 2019;       Accepted: Oct. 4, 2019;       Published: Sep. 3, 2020
DOI: 10.11648/j.ijfet.20200402.14      View  168      Downloads  57
Abstract
A liquid chromatographic tandem mass spectrometric (HPLC MS/MS) method is proposed for identification and quantification of antibiotics residues and metabolites in honey and other matrices such as water, manure, soil, meat and sediments. This method was used to detect tetracycline derived residues in honey samples from Tabora and Singida regions, Tanzania. This honey was obtained from modern beehives. Three hundred honey samples were tested for the presence of tetracycline derivative residues. Of the examined samples 15% had these drug residues. Majority of samples contained residues to a proportion of 0.002–0.008 mg/kg. The findings show that tetracycline antibiotic residues are present in honey from Tabora and Singida regions, although in small concentrations, however, this can possibly influence the naturally occurring protective properties of this medicinal food. Also can lead to drug resistance to consumers. Prospective studies that simultaneously track both resistance genes and antibiotic residues will go far in resolving some of the gagging questions that cloud our understanding of antibiotic resistance dissemination.
Keywords
Honey, Tetracycline Residues, Mass Spectrometry, Tabora, Singida, Tanzania
To cite this article
Mohamed Hamed Sango Ally, Mnyakavilli Anna, Determination of Tetracycline Residues in Honey from Tabora and Singida Regions Tanzania Produced Using Modern Beehives, International Journal of Food Engineering and Technology. Vol. 4, No. 2, 2020, pp. 32-35. doi: 10.11648/j.ijfet.20200402.14
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Batt PJ, Liu A 2012. Consumer behavior towards honey products in western Australia. Brit Food J. 114. 285-297.
[2]
Ceyhan H, Ugur A, 2001. Investigation of in vitro antimicrobial activity of honey. Review in Biology 94, 363-371.
[3]
Dixion B, 2003. Bacteria can not resist honey. Lancent Infectious diseases 2, p116.
[4]
Evans J. D. 2003. Diverse origins of tetracycline resistance in honey bee bacterial pathogen Paenibacillus larvae. Journal of International Pathology, 83, 46-50.
[5]
Heering W, Usleber E, Dietrich R and Martlbauer E, 1998. Immunochemical screening for antimicrobial drugs residues in commercial honey. Analyst 123, 275 –2762.
[6]
Kaufmann A, Roth S, Ryser B, Widmer M and Gruggisberg D 2002. Quantitative LC MS MS determination of sulfonamides and some other antibiotics in honey. Journal of AOAC International, 85, 853 860.
[7]
Molan P. C, 2002. Re introducing honey in the management of wounds, ulcers, theory and practice. Ostomy/wound management, 48, 28-40.
[8]
Muli E, Munguti A, Raina SK (2007. Quality of Honey Harvested and Processed Using Traditional Methods in Rural Areas of Kenya. Acta Vet Brno, 76. 315-320.
[9]
Ngaga Y. M, Otsyana R, Senkondo E and Mpuya P 2005. Economic Survey on the role of beekeeping on poverty reduction and environmental conservation in Chunya, Songea and Nachingwea districts, Tanzania.
[10]
Oka H, Ikai Y, Kawamura N, Unok K, Yamada M, Harrada K, and Suzuki M, 1987. Improvement of chemical analysis of antibiotics XII. Simultaneous analysis of seven tetracyclines in honey. Journal of Chromatography, 400, 253-261.
[11]
Taormina P. J, Niemira B. A, Benchat L. R 2001. Inhibitory activity of honey against food borne pathogens as influenced by the presence of hydrogen peroxide and level of antioxdant power. International Journal of Food Microbiology, 69, 217-225.
[12]
Tichy J and Novak J, 2000. Detection of antimicrobials in bee products with activity against viridans streptococci. Journal of Alternative and Complementary Medicine, 6, 383-389.
[13]
Verzegnessi L, Royer D, Mottier P and Stadler RH 2003. Analysis of chlorompenicol in honeys of different geographical origins by liquid chromatography coupled to electrospray ionisation tandem mass spectrometry. Food additives and Contaminants, 20, 335-342.
[14]
Vinas P, Balsalobre N, Lopez Erroz C, Hernandez Cordoba M, 2004. Liquid chromatography with ultraviolet absorbance detection for the analysis of tetracycline residues in honey. Journal of chromatographic Analysis, 1022, 125-129.
[15]
Wang J. 2004. Confirmatory determination of six penicllins in honey by liquid chromatography/eletrospray ionisation tandem mass spectrometry. Journal AOAC International, 187, 45-55.
[16]
Zaghloul A. A, El shattawy H. H, Kassem AA, Ibrahim EA, Reddy IK and Khan MA 2001. Honey, a perspective antibiotic, Extraction, formulation and stability. Die Pharmazie, 56, 643-647.
Browse journals by subject