Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells

Fernando Candanedo-Gonzalez; Octavio Galindo-Hernandez; Nathalia Serna-Marquez; Roberto Espinosa-Neira; Adriana Soto-Guzman; Pedro Cortes-Reynosa; Eduardo Perez Salazar

doi:doi:10.11648/j.crj.20140204.11

Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells

Fernando Candanedo-Gonzalez, Octavio Galindo-Hernandez, Nathalia Serna-Marquez, Roberto Espinosa-Neira, Adriana Soto-Guzman, Pedro Cortes-Reynosa, Eduardo Perez Salazar

Published in Cancer Research Journal (Volume 2, Issue 4)

Received: 13 June 2014 Accepted: 28 June 2014 Published: 10 July 2014

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Abstract

Progesterone (PG) is a steroid hormone that regulates normal reproductive functions including uterine and mammary gland development. The PG receptor belongs to the nuclear receptor superfamily of ligand dependent transcription factors that mediates gene expression, however, it also promotes cell signaling pathways through a non-genomic pathway including the activation of PI3K/Akt and Src/ERK1/2 pathways. However the role of PG in NFκB-DNA complex formation remains to be studied. We demonstrate here that PG induces Akt2 activation and an increase of NFκB DNA binding activity in MCF-7 breast cancer cells. Akt2 activation requires PI3K activity, whereas increase of NFκB DNA binding activity requires PI3K, mTOR, Akt, Src, and G-proteins activity, as well as the integrity of cytoskeleton. In summary, our findings demonstrate that PG induces an increase of NFκB DNA binding activity in MCF-7 cells.

Published in	Cancer Research Journal (Volume 2, Issue 4)
DOI	10.11648/j.crj.20140204.11
Page(s)	63-69
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Breast Cancer, Progesterone, PI3K/Akt, NFκB, MCF-7

References

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APA Style

Fernando Candanedo-Gonzalez, Octavio Galindo-Hernandez, Nathalia Serna-Marquez, Roberto Espinosa-Neira, Adriana Soto-Guzman, et al. (2014). Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells. Cancer Research Journal, 2(4), 63-69. https://doi.org/10.11648/j.crj.20140204.11

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ACS Style

Fernando Candanedo-Gonzalez; Octavio Galindo-Hernandez; Nathalia Serna-Marquez; Roberto Espinosa-Neira; Adriana Soto-Guzman, et al. Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells. Cancer Res. J. 2014, 2(4), 63-69. doi: 10.11648/j.crj.20140204.11

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AMA Style

Fernando Candanedo-Gonzalez, Octavio Galindo-Hernandez, Nathalia Serna-Marquez, Roberto Espinosa-Neira, Adriana Soto-Guzman, et al. Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells. Cancer Res J. 2014;2(4):63-69. doi: 10.11648/j.crj.20140204.11

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@article{10.11648/j.crj.20140204.11,
  author = {Fernando Candanedo-Gonzalez and Octavio Galindo-Hernandez and Nathalia Serna-Marquez and Roberto Espinosa-Neira and Adriana Soto-Guzman and Pedro Cortes-Reynosa and Eduardo Perez Salazar},
  title = {Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells},
  journal = {Cancer Research Journal},
  volume = {2},
  number = {4},
  pages = {63-69},
  doi = {10.11648/j.crj.20140204.11},
  url = {https://doi.org/10.11648/j.crj.20140204.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20140204.11},
  abstract = {Progesterone (PG) is a steroid hormone that regulates normal reproductive functions including uterine and mammary gland development. The PG receptor belongs to the nuclear receptor superfamily of ligand dependent transcription factors that mediates gene expression, however, it also promotes cell signaling pathways through a non-genomic pathway including the activation of PI3K/Akt and Src/ERK1/2 pathways. However the role of PG in NFκB-DNA complex formation remains to be studied. We demonstrate here that PG induces Akt2 activation and an increase of NFκB DNA binding activity in MCF-7 breast cancer cells. Akt2 activation requires PI3K activity, whereas increase of NFκB DNA binding activity requires PI3K, mTOR, Akt, Src, and G-proteins activity, as well as the integrity of cytoskeleton. In summary, our findings demonstrate that PG induces an increase of NFκB DNA binding activity in MCF-7 cells.},
 year = {2014}
}

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TY  - JOUR
T1  - Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells
AU  - Fernando Candanedo-Gonzalez
AU  - Octavio Galindo-Hernandez
AU  - Nathalia Serna-Marquez
AU  - Roberto Espinosa-Neira
AU  - Adriana Soto-Guzman
AU  - Pedro Cortes-Reynosa
AU  - Eduardo Perez Salazar
Y1  - 2014/07/10
PY  - 2014
N1  - https://doi.org/10.11648/j.crj.20140204.11
DO  - 10.11648/j.crj.20140204.11
T2  - Cancer Research Journal
JF  - Cancer Research Journal
JO  - Cancer Research Journal
SP  - 63
EP  - 69
PB  - Science Publishing Group
SN  - 2330-8214
UR  - https://doi.org/10.11648/j.crj.20140204.11
AB  - Progesterone (PG) is a steroid hormone that regulates normal reproductive functions including uterine and mammary gland development. The PG receptor belongs to the nuclear receptor superfamily of ligand dependent transcription factors that mediates gene expression, however, it also promotes cell signaling pathways through a non-genomic pathway including the activation of PI3K/Akt and Src/ERK1/2 pathways. However the role of PG in NFκB-DNA complex formation remains to be studied. We demonstrate here that PG induces Akt2 activation and an increase of NFκB DNA binding activity in MCF-7 breast cancer cells. Akt2 activation requires PI3K activity, whereas increase of NFκB DNA binding activity requires PI3K, mTOR, Akt, Src, and G-proteins activity, as well as the integrity of cytoskeleton. In summary, our findings demonstrate that PG induces an increase of NFκB DNA binding activity in MCF-7 cells.
VL  - 2
IS  - 4
ER  -

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Author Information

Fernando Candanedo-Gonzalez

Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
Octavio Galindo-Hernandez

Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
Nathalia Serna-Marquez

Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
Roberto Espinosa-Neira

Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
Adriana Soto-Guzman

Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
Pedro Cortes-Reynosa

Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico
Eduardo Perez Salazar

Departamento de Biologia Celular. Cinvestav-IPN. Av IPN # 2508. San Pedro Zacatenco. Mexico, DF. 07360. Mexico

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APA Style

Fernando Candanedo-Gonzalez, Octavio Galindo-Hernandez, Nathalia Serna-Marquez, Roberto Espinosa-Neira, Adriana Soto-Guzman, et al. (2014). Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells. Cancer Research Journal, 2(4), 63-69. https://doi.org/10.11648/j.crj.20140204.11

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ACS Style

Fernando Candanedo-Gonzalez; Octavio Galindo-Hernandez; Nathalia Serna-Marquez; Roberto Espinosa-Neira; Adriana Soto-Guzman, et al. Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells. Cancer Res. J. 2014, 2(4), 63-69. doi: 10.11648/j.crj.20140204.11

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AMA Style

Fernando Candanedo-Gonzalez, Octavio Galindo-Hernandez, Nathalia Serna-Marquez, Roberto Espinosa-Neira, Adriana Soto-Guzman, et al. Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells. Cancer Res J. 2014;2(4):63-69. doi: 10.11648/j.crj.20140204.11

Copy | Download

@article{10.11648/j.crj.20140204.11,
  author = {Fernando Candanedo-Gonzalez and Octavio Galindo-Hernandez and Nathalia Serna-Marquez and Roberto Espinosa-Neira and Adriana Soto-Guzman and Pedro Cortes-Reynosa and Eduardo Perez Salazar},
  title = {Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells},
  journal = {Cancer Research Journal},
  volume = {2},
  number = {4},
  pages = {63-69},
  doi = {10.11648/j.crj.20140204.11},
  url = {https://doi.org/10.11648/j.crj.20140204.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20140204.11},
  abstract = {Progesterone (PG) is a steroid hormone that regulates normal reproductive functions including uterine and mammary gland development. The PG receptor belongs to the nuclear receptor superfamily of ligand dependent transcription factors that mediates gene expression, however, it also promotes cell signaling pathways through a non-genomic pathway including the activation of PI3K/Akt and Src/ERK1/2 pathways. However the role of PG in NFκB-DNA complex formation remains to be studied. We demonstrate here that PG induces Akt2 activation and an increase of NFκB DNA binding activity in MCF-7 breast cancer cells. Akt2 activation requires PI3K activity, whereas increase of NFκB DNA binding activity requires PI3K, mTOR, Akt, Src, and G-proteins activity, as well as the integrity of cytoskeleton. In summary, our findings demonstrate that PG induces an increase of NFκB DNA binding activity in MCF-7 cells.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Progesterone Induces NFκB DNA Binding Activity through a PI3K/Akt-Dependent Pathway in MCF-7 Breast Cancer Cells
AU  - Fernando Candanedo-Gonzalez
AU  - Octavio Galindo-Hernandez
AU  - Nathalia Serna-Marquez
AU  - Roberto Espinosa-Neira
AU  - Adriana Soto-Guzman
AU  - Pedro Cortes-Reynosa
AU  - Eduardo Perez Salazar
Y1  - 2014/07/10
PY  - 2014
N1  - https://doi.org/10.11648/j.crj.20140204.11
DO  - 10.11648/j.crj.20140204.11
T2  - Cancer Research Journal
JF  - Cancer Research Journal
JO  - Cancer Research Journal
SP  - 63
EP  - 69
PB  - Science Publishing Group
SN  - 2330-8214
UR  - https://doi.org/10.11648/j.crj.20140204.11
AB  - Progesterone (PG) is a steroid hormone that regulates normal reproductive functions including uterine and mammary gland development. The PG receptor belongs to the nuclear receptor superfamily of ligand dependent transcription factors that mediates gene expression, however, it also promotes cell signaling pathways through a non-genomic pathway including the activation of PI3K/Akt and Src/ERK1/2 pathways. However the role of PG in NFκB-DNA complex formation remains to be studied. We demonstrate here that PG induces Akt2 activation and an increase of NFκB DNA binding activity in MCF-7 breast cancer cells. Akt2 activation requires PI3K activity, whereas increase of NFκB DNA binding activity requires PI3K, mTOR, Akt, Src, and G-proteins activity, as well as the integrity of cytoskeleton. In summary, our findings demonstrate that PG induces an increase of NFκB DNA binding activity in MCF-7 cells.
VL  - 2
IS  - 4
ER  -

Copy | Download